| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| Other Sizes |
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| ln Vitro |
In a dosage-dependent manner, 2OH-BNPP1 (0.1-50 μM) eliminates TGFβ signaling. In both normal cell lines and many cancer cell lines, 2OH-BNPP1 dose-dependently reduces TGFβ-induced phosphorylation of proteins that mediate canonical and non-canonical TGFβ signaling [1].
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| ln Vivo |
In vivo TGFβ signaling is blocked by 2OH-BNPP1 (50 mg/kg), and phosphorylated SMAD2 abundance is markedly decreased in tumor-bearing animals [1].
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| References |
[1]. Nyati S, et al. The kinase activity of the Ser/Thr kinase BUB1 promotes TGF-β signaling. Sci Signal. 2015 Jan 6;8(358):ra1
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| Molecular Formula |
C16H19N5O
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|---|---|
| Molecular Weight |
297.36
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| Exact Mass |
297.159
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| CAS # |
833481-73-5
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| PubChem CID |
59796535
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| Appearance |
Typically exists as solid at room temperature
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| LogP |
3.041
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
22
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| Complexity |
385
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
LWLCFWPPMNPMOI-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C16H19N5O/c1-16(2,3)21-15-13(14(17)18-9-19-15)11(20-21)8-10-6-4-5-7-12(10)22/h4-7,9,22H,8H2,1-3H3,(H2,17,18,19)
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| Chemical Name |
2-[(4-amino-1-tert-butylpyrazolo[3,4-d]pyrimidin-3-yl)methyl]phenol
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| Synonyms |
2OH-BNPP1 2OH BNPP1 2OHBNPP1
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ≥ 100 mg/mL (~336.30 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (8.41 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.3629 mL | 16.8146 mL | 33.6293 mL | |
| 5 mM | 0.6726 mL | 3.3629 mL | 6.7259 mL | |
| 10 mM | 0.3363 mL | 1.6815 mL | 3.3629 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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