Size | Price | Stock | Qty |
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5mg |
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10mg |
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25mg |
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50mg |
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100mg |
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250mg |
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Other Sizes |
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Targets |
COLO 205 (IC50 = 9.0 μM); HCT-116 (IC50 = 40.2 μM); HT-29 (IC50 = 70.9 μM)
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ln Vitro |
3'-Hydroxypterostilbene decreases cell growth in cultured human colon cancer cells (COLO 205, HCT-116, and HT-29) in a dose-dependent manner, with IC50 values of 9.0, 40.2, and 70.9 µM, respectively. By inducing apoptosis and autophagy, 3'-hydroxypterostilbene successfully slows the growth of human colon cancer cells. Prior to the onset of apoptosis, treatment with HPSB reduces the potential of the mitochondrial membrane and induces caspase-3 and caspage-9, which are linked to the breakdown of DFF-45 and PARP. It significantly reduces phosphatidylinositol 3-kinase (PI3K)/Akt, mitogen-activated protein kinases (MAPKs), and phosphatidylinositol 3-kinase (mTOR) signaling, among other things[1].
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ln Vivo |
Through the inhibition of inflammation, metastasis, and angiogenesis as well as through the induction of apoptosis, 3'-Hydroxypterostilbene by intravenous injection has anti-tumor efficacy against colon cancer[1].
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Cell Assay |
COLO 205 cells (2*105 cells/mL) are cultured in 12 wells and exposed to varying concentrations of pterostilbene or 3′-hydroxypterostilbene for 24 hours in order to analyze the sub-G1 cell population. The cells are subsequently removed, PBS-washed, re-suspended in 200 µL of PBS, and fixed in 800 µL of ice-cold 100% ethanol at -20 degrees Celsius. The cell pellets are centrifuged after being allowed to stand overnight, resuspended in 1 mL of hypotonic buffer, which contains PI (50 µg/mL), 0.5% Triton X-100 in PBS, and 0.5 µg/mL RNase. This process takes 30 minutes at 37°C in the dark. After the fluorescent dye has been excited, the PI-DNA complex's fluorescence is quantitated.
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References |
Molecular Formula |
C16H16O4
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Molecular Weight |
272.2958
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Exact Mass |
272.10485899
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CAS # |
475231-21-1
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Related CAS # |
475231-21-1
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Appearance |
Solid
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SMILES |
COC1=CC(=CC(=C1)/C=C/C2=CC(=C(C=C2)O)O)OC
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InChi Key |
UQRBFXIUUDJHSN-ONEGZZNKSA-N
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InChi Code |
InChI=1S/C16H16O4/c1-19-13-7-12(8-14(10-13)20-2)4-3-11-5-6-15(17)16(18)9-11/h3-10,17-18H,1-2H3/b4-3+
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Chemical Name |
4-[(E)-2-(3,5-dimethoxyphenyl)ethenyl]benzene-1,2-diol
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Synonyms |
3'-Hydroxypterostilbene
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO: 54~125 mg/mL (198.3~459.1 mM)
Ethanol: 54 mg/mL (~198.3 mM) |
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (7.64 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (7.64 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.08 mg/mL (7.64 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 3.6724 mL | 18.3621 mL | 36.7242 mL | |
5 mM | 0.7345 mL | 3.6724 mL | 7.3448 mL | |
10 mM | 0.3672 mL | 1.8362 mL | 3.6724 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Pterostilbene and 3′-hydroxypterostilbene induced apoptosis in COLO205 cancer cells. PLoS One . 2014 Nov 12;9(11):e111814. td> |
Autophagy induction by pterostilbene and 3′-hydroxypterostilbene in COLO 205 cancer cells. PLoS One . 2014 Nov 12;9(11):e111814. td> |
3′-Hydroxypterostilbene down-regulated mTOR, PI3K/Akt and MAPKs signaling in COLO 205 cancer cells. PLoS One . 2014 Nov 12;9(11):e111814. td> |
Autophagy inhibitor sensitized pterostilbene and 3′-hydroxypterostilbene-induced apoptosis in COLO 205 cancer cells. PLoS One . 2014 Nov 12;9(11):e111814. td> |
Pterostilbene and 3′-hydroxypterostilbene reduced the growth of COLO 205 xenografts in nude mice. PLoS One . 2014 Nov 12;9(11):e111814. td> |