Size | Price | Stock | Qty |
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500mg |
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1g |
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5g |
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Other Sizes |
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ln Vitro |
4-Methylbenzylidenecamphor (4-MBC; 5-400 μM; 48 hours) suppresses the growth of HTR8/SVneo cells [1]. Human trophoblast cell proliferation is induced by 4-methylbenzylidene camphor (10–50 μM; 48 hours) and results in an increase in the percentage of cells in the SubG1 phase [1]. 4-In 48 hours, 50 μM methylbenzylidenecamphor decreases human trophoblast cells' nutrition [1]. In human trophoblast cells, camphor (50 μM; 5-120 minutes) stimulates the signal amplifiers for PI3K/AKT and ERK1/2 [1]. 4-Methylbenzylidene camphor at 20–50 μM for 24 hours dramatically raises GPR56 and SEMA6 A.
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ln Vivo |
Changes in gene expression and South African growth enhancement in newborns are caused by 4-Methylbenzylidenecamphor (4-MBC; 0.7, 7, 24, 47 mg/kg/day; given to parents in diet prior to mating, during pregnancy, and during fetal formulation) [2].
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Cell Assay |
cell proliferation assay [1]
Cell Types: HTR8/SVneo Cell Tested Concentrations: 0, 5, 10, 20, 50, 100 , 200 and 400 μM Incubation Duration: 48 h Experimental Results: Dose-dependently inhibited cell proliferation of HTR8/SVneo cell. Apoptosis analysis [1] Cell Types: HTR8/SVneo Cell Tested Concentrations: 10, 20, 50 μM Incubation Duration: 48 h Experimental Results: Early and late apoptotic cells increased Dramatically at 20 μM and 50 μM. Cell cycle analysis [1] Cell Types: HTR8/SVneo Cell Tested Concentrations: 5, 10, 20, 50 μM Incubation Duration: 48 h Experimental Results: The proportion of cells in the SubG1 phase gradually increased. Cell Invasion Analysis[1] Cell Types: HTR8/SVneo Cell Tested Concentrations: 50 μM Incubation Duration: 48 hrs (hours) Experimental Results: Invasiveness was Dramatically diminished by 81.5% Western Blot Analysis[1] Cell Types: HTR8/SVneo Cell Tested Concentrations: 50 μM Incubation Duration: 0, 5, 15, 30, 60, 120 minutes Experimental Results: The phosphorylation of AKT and its downstream kinase protein P70S6K peaked at 5 and 15 minutes respectively, then diminished after 30 minutes, an |
References |
[1]. Changwon Yang, et al. 4-Methylbenzylidene-camphor inhibits proliferation and induces reactive oxygen species-mediated apoptosis of human trophoblast cells. Reprod Toxicol. 2019 Mar:84:49-58.
[2]. Margret Schlumpf, et al. Developmental toxicity of UV filters and environmental exposure: a review. Int J Androl. 2008 Apr;31(2):144-51. |
Molecular Formula |
C18H22O
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Molecular Weight |
254.3667
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CAS # |
36861-47-9
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Related CAS # |
4-Methylbenzylidene camphor-d4;1219806-41-3
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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SMILES |
O=C1/C(=C(\[H])/C2C([H])=C([H])C(C([H])([H])[H])=C([H])C=2[H])/[C@@]2([H])C([H])([H])C([H])([H])C1(C([H])([H])[H])C2(C([H])([H])[H])C([H])([H])[H]
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : ~100 mg/mL (~393.13 mM)
H2O : < 0.1 mg/mL |
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Solubility (In Vivo) |
Solubility in Formulation 1: 2.5 mg/mL (9.83 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 2.5 mg/mL (9.83 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (9.83 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 3.9313 mL | 19.6564 mL | 39.3128 mL | |
5 mM | 0.7863 mL | 3.9313 mL | 7.8626 mL | |
10 mM | 0.3931 mL | 1.9656 mL | 3.9313 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.