| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
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| Other Sizes |
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| ln Vitro |
Advice (This is our suggested protocol, which should be adjusted to suit your particular needs as it only offers guidance). Assay for serum acid phosphatase[1]: 1. Mix 50 μL of 4-methylumbelliferate phosphate (5.0 mM), 10 μL of 1.0 M pH 6.0 buffer, and 5.0 μL of serum enzyme into a solution to make 100 μL. 2. Include the following reagents (final concentrations): 45 mM EDTA, 6 mM EGTA, 4 mM 1,10-phenanthroline, 6 mM 8 quinolinol, 6 mM 8-hydroxy-5-quinolinesulfonic acid, 20 mM sodium tartrate, 15 mM sodium fluoride, and 3000 mM 2-mercaptoethanol. 3. To stop the reaction, add 2.9 mL of 0.1 M ammonium hydroxide/glycine buffer (pH 10.5) to the reaction mixture and incubate it for 15 minutes at 37°C. 4. Using a fluorometer, measure the fluorescence after thoroughly mixing the sample.
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|---|---|
| References |
|
| Molecular Formula |
C10H9O6P
|
|---|---|
| Molecular Weight |
256.14866
|
| Exact Mass |
240.018
|
| CAS # |
3368-04-5
|
| Related CAS # |
4-Methylumbelliferyl phosphate disodium;22919-26-2
|
| PubChem CID |
65118
|
| Appearance |
White to off-white solid powder
|
| Density |
1.6±0.1 g/cm3
|
| Boiling Point |
513.6±60.0 °C at 760 mmHg
|
| Melting Point |
215-218 °C
|
| Flash Point |
264.4±32.9 °C
|
| Vapour Pressure |
0.0±1.4 mmHg at 25°C
|
| Index of Refraction |
1.628
|
| LogP |
0.75
|
| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
6
|
| Rotatable Bond Count |
2
|
| Heavy Atom Count |
17
|
| Complexity |
398
|
| Defined Atom Stereocenter Count |
0
|
| InChi Key |
BCHIXGBGRHLSBE-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C10H9O6P/c1-6-4-10(11)15-9-5-7(2-3-8(6)9)16-17(12,13)14/h2-5H,1H3,(H2,12,13,14)
|
| Chemical Name |
(4-methyl-2-oxochromen-7-yl) dihydrogen phosphate
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
H2O : ~20.83 mg/mL (~81.32 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 10 mg/mL (39.04 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.9040 mL | 19.5198 mL | 39.0396 mL | |
| 5 mM | 0.7808 mL | 3.9040 mL | 7.8079 mL | |
| 10 mM | 0.3904 mL | 1.9520 mL | 3.9040 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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