| Size | Price | Stock | Qty |
|---|---|---|---|
| 50mg |
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| 100mg |
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| Other Sizes |
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| ln Vitro |
Preparation of 5(6)-CFDA working solution 1. Preparation of stock solution Dissolve 1 milligram 5(6)-CFDA in 0.21 mL DMSO to yield 10 mM 5(6)-CFDA. Note: It is advised that the stock solution be in - 2. For 5(6)-CFDA working solution, utilize serum-free cell solution or PBS extraction solution to obtain 1-10 μM 5(6)-CFDA working solution. Store at 20℃, -80℃ away from light and avoid repeated freezing and thawing. Note: Please adjust the concentration of 5(6)-CFDA working solution according to the real situation. Cell staining 1. Cell preparation: Suspension cells: Centrifuge at 1000g for 3-5 minutes at 4°C, then discard the supernatant. Use PBS to wash twice, for five minutes each time. Adherent cells: Discard the decellularized cells and add the islet dissociated cells to produce a single cell solution. Discard the supernatant after centrifuging at 1000g for three to five minutes at 4°C. Use PBS to wash twice, for five minutes each time. 2. Add 1 mL of 5(6)-CFDA working solution and continue staining for 30 minutes. 3. Centrifuge at 400g for 3-4 minutes at 4°C and discard the supernatant. 4. Use PBS to wash twice, for five minutes each time. 5. Resuspend cells in serum-free cell buffer or PBS, and then detect using fluorescence microscopy or flow cytometer. Observations 1. It is advised that the reserve liquid be stored at -20°C or - 2. Please adjust the concentration of 5(6)-CFDA working solution according to the real situation. 3. This product is for research and development use only and is not intended for pharmaceutical, home or other purposes. 4. For your safety and health, please wear a white coat and gloves when operating.
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| References |
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| Molecular Formula |
2[C25H16O9]
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|---|---|
| Molecular Weight |
920.778280000001
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| Exact Mass |
920.158
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| CAS # |
124387-19-5
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| Related CAS # |
5-CFDA;79955-27-4;6-CFDA;3348-03-6
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| PubChem CID |
44119974
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| Appearance |
White to yellow solid powder
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| LogP |
7.606
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
18
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| Rotatable Bond Count |
10
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| Heavy Atom Count |
68
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| Complexity |
1660
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
SWRGCNMDGRMQGB-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/2C25H16O9/c1-12(26)31-15-4-7-18-21(10-15)33-22-11-16(32-13(2)27)5-8-19(22)25(18)20-9-14(23(28)29)3-6-17(20)24(30)34-25;1-12(26)31-14-6-8-18-20(10-14)33-21-11-15(32-13(2)27)7-9-19(21)25(18)22-16(23(28)29)4-3-5-17(22)24(30)34-25/h2*3-11H,1-2H3,(H,28,29)
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| Chemical Name |
3',6'-diacetyloxy-1-oxospiro[2-benzofuran-3,9'-xanthene]-4-carboxylic acid;3',6'-diacetyloxy-1-oxospiro[2-benzofuran-3,9'-xanthene]-5-carboxylic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~125 mg/mL (~271.51 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (4.52 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (4.52 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.0860 mL | 5.4302 mL | 10.8604 mL | |
| 5 mM | 0.2172 mL | 1.0860 mL | 2.1721 mL | |
| 10 mM | 0.1086 mL | 0.5430 mL | 1.0860 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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