| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
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| 500mg |
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| 1g |
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| Other Sizes |
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| ln Vitro |
5-LOX-IN-2 (Compound 10b) has a cytotoxic effect on renal cancer cells and is specific to RCC4 and 786.0 cells that lack the Von Hippel-Lindau (VHL) inhibitory oncogene. In RCC4 cells, 5-LOX-IN-2 (Compound 10b) (0-10 μM; 24 hours) can also upregulate p62 and LC3B expression, inhibit autophagy carriers, and promote cell proliferation through activation of the caspase-3 pathway. 1].
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|---|---|
| Cell Assay |
Cell viability assay [1]
Cell Types: RCC4, RCC10, 786.0 Cell Tested Concentrations: 0-100 μM Incubation Duration: 4 days Experimental Results: The cell viability of renal cancer cells is diminished and the selectivity is stronger. RCC4 and 786.0 cells lack the Von Hippel-Lindau (VHL) tumor suppressor gene. Western Blot Analysis[1] Cell Types: RCC4, RCC10, 786.0 Cell Tested Concentrations: 0-10 μM Incubation Duration: 24 hrs (hours) Experimental Results: LC3B and p62 expression increased and autophagy flow was blocked in RCC4 cells. Cleavage of caspase-3 precursors was stimulated in a dose-dependent manner only in RCC4 cells lacking the VHL tumor suppressor. |
| References |
| Molecular Formula |
C17H16O4
|
|---|---|
| Molecular Weight |
284.31
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| Exact Mass |
284.104
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| Elemental Analysis |
C, 71.82; H, 5.67; O, 22.51
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| CAS # |
179691-97-5
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| PubChem CID |
5909099
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| Appearance |
Off-white to light yellow solid powder
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| Density |
1.3±0.1 g/cm3
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| Boiling Point |
515.5±50.0 °C at 760 mmHg
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| Flash Point |
192.2±23.6 °C
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| Vapour Pressure |
0.0±1.4 mmHg at 25°C
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| Index of Refraction |
1.646
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| LogP |
3.7
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
6
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| Heavy Atom Count |
21
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| Complexity |
347
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C1=CC=C(C=C1)CCOC(=O)/C=C/C2=C(C=CC(=C2)O)O
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| InChi Key |
OQKRMXDGEFRBAJ-RMKNXTFCSA-N
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| InChi Code |
InChI=1S/C17H16O4/c18-15-7-8-16(19)14(12-15)6-9-17(20)21-11-10-13-4-2-1-3-5-13/h1-9,12,18-19H,10-11H2/b9-6+
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| Chemical Name |
phenethyl (E)-3-(2,5-dihydroxyphenyl)acrylate
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| Synonyms |
NSC666588; CHEMBL131678; NSC-666588; 5LOX-IN-2; 5-LOX-IN2; 5-LOX-IN-2
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~351.73 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (8.79 mM) (saturation unknown) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.5173 mL | 17.5864 mL | 35.1729 mL | |
| 5 mM | 0.7035 mL | 3.5173 mL | 7.0346 mL | |
| 10 mM | 0.3517 mL | 1.7586 mL | 3.5173 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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