| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
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| 500mg |
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| Other Sizes |
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| ln Vitro |
5-FAM includes a carboxylic acid that is activated by carbodiimide when it comes into contact with primary amines. The most popular fluorescent derivatization reagent for labeling biomolecules is fluorescein. Apart from its exceptional fluorescence quantum yield, superior water solubility, and relatively high absorption rate, fluorescein also exhibits an excitation maximum that is quite similar to the argon ion laser's 488 nm spectral line [1].
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|---|---|
| References | |
| Additional Infomation |
5-carboxyfluorescein is a monocarboxylic acid. It has a role as a fluorochrome. It is functionally related to a fluorescein (lactone form).
|
| Molecular Formula |
C21H12O7
|
|---|---|
| Molecular Weight |
376.32
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| Exact Mass |
376.058
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| CAS # |
76823-03-5
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| Related CAS # |
5-FAM SE;92557-80-7
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| PubChem CID |
123755
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| Appearance |
Light yellow to yellow solid powder
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| Density |
1.7±0.1 g/cm3
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| Boiling Point |
725.2±60.0 °C at 760 mmHg
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| Melting Point |
368-372°C
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| Flash Point |
266.9±26.4 °C
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| Vapour Pressure |
0.0±2.5 mmHg at 25°C
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| Index of Refraction |
1.816
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| LogP |
2.62
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
7
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
28
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| Complexity |
637
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
NJYVEMPWNAYQQN-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C21H12O7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)21(15)14-4-1-10(19(24)25)7-13(14)20(26)28-21/h1-9,22-23H,(H,24,25)
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| Chemical Name |
5-Carboxyfluorescein 3',6'-Dihydroxy-3-oxo-spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-5-carboxylic acid
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| Synonyms |
5-FAM 5-Carboxyfluorescein 5 Carboxyfluorescein 5Carboxyfluorescein5 FAM 5FAM
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ≥ 31 mg/mL (~82.38 mM)
H2O : ~1 mg/mL (~2.66 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (6.64 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (6.64 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.6573 mL | 13.2866 mL | 26.5731 mL | |
| 5 mM | 0.5315 mL | 2.6573 mL | 5.3146 mL | |
| 10 mM | 0.2657 mL | 1.3287 mL | 2.6573 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
| NCT Number | Recruitment | interventions | Conditions | Sponsor/Collaborators | Start Date | Phases |
| NCT03850132 | COMPLETED | Behavioral: FAM-SOTC-PL | Bereavement | University of Iceland | 2014-07-27 | Not Applicable |
| NCT02815462 | WITHDRAWN | Other: FAMFACESG Other: Control Other: FAMFACESG |
Patient Readmission | Singapore General Hospital | 2016-08 | Not Applicable |
| NCT03901027 | COMPLETED | Behavioral: Therapeutic Conversation Interventions | Chronic Illnesses | University of Iceland | 2015-03-01 | Not Applicable |
| NCT06114186 | RECRUITING | Device: FAM intervention consisting of a Follower of glucose data, Action Plan, and Remote Monitoring of glucose data Other: standard of care |
Type 1 Diabetes | University of Minnesota | 2024-01-16 | Early Phase 1 |
| NCT04339465 | UNKNOWN STATUS | Behavioral: CARE-FAM Behavioral: WEP-CARE |
Rare Diseases | Silke Wiegand-Grefe, Prof. Dr. | 2019-01-01 | Not Applicable |
Products are for research use only; We do not sell to patients
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