| Size | Price | |
|---|---|---|
| 500mg | ||
| 1g | ||
| Other Sizes |
| ln Vitro |
6-O-Isobutyrylbritannilactone (5-100 μM; 48 h) in comparison to IBMX (100 μM; 24 h)[1]. 6-O-isobutyrylbritannilactone (5-30 μM; 12 h) suppresses albino-related mRNA levels in B16F10 cells treated with IBMX (100 μM; 48 h) in a dose-dependent manner[1]. 6-O-isobutyrylbritannilactone (20 μM; 1-9 h) suppresses phosphorylation of ERK, AKT, and CREB in a time-dependent manner [1].
|
|---|---|
| ln Vivo |
In comparison to untreated controls, 6-O-isobutyrylbritannilactone (10-100 μM; 48 h) dramatically lowers pigmentation in zebrafish embryos [1].
|
| Cell Assay |
RT-PCR[1]
Cell Types: B16F10 cells Tested Concentrations: 5 μM, 10 μM, 20 μM, 30 μM Incubation Duration: 12 hrs (hours); with 100 μM IBMX for 48 hrs (hours) Experimental Results: Inhibited whitening-related mRNA levels, and resulted in completely inhibited expressions of melanogenesis-related protein levels at 20 μM. Western Blot Analysis[1] Cell Types: 20 μM Tested Concentrations: 5 μM, 10 μM, 20 μM, 30 μM Incubation Duration: 1 h, 3 h, 6 h, and 9 h Experimental Results: Inhibited the phosphorylation of ERK, AKT, and CREB. Demonstrated the melanogenesis suppression induced by IBMX via inaction of multiple signaling pathways. Cell Cytotoxicity Assay[1] Cell Types: B16F10 cells Tested Concentrations: 5 μM, 10 μM, 20 μM, 50 μM, and 100 μM Incubation Duration: 24 hrs (hours) Experimental Results: Inhibited B16F10 cells viability in a dose-dependent manner. |
| Animal Protocol |
Animal/Disease Models: Zebrafish embryos[1]
Doses: 10 μM, 50 μM, and 100 μM Route of Administration: Dipped in culture for 48 hrs (hours) Experimental Results: diminished pigmentation by approximately 8%, 13%, and 16%. |
| References | |
| Additional Infomation |
(3aR)-3-Methylene-4alpha-(isobutyryloxy)-5beta-[(S)-1-methyl-4-hydroxybutyl]-6-methyl-2,3,3aalpha,4,7,7aalpha-hexahydrobenzofuran-2-one has been reported in Inula japonica and Pentanema britannicum with data available.
|
| Molecular Formula |
C19H28O5
|
|---|---|
| Molecular Weight |
336.42
|
| Exact Mass |
336.193
|
| CAS # |
1259933-02-2
|
| PubChem CID |
50917934
|
| Appearance |
Typically exists as solid at room temperature
|
| LogP |
2.4
|
| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
5
|
| Rotatable Bond Count |
7
|
| Heavy Atom Count |
24
|
| Complexity |
560
|
| Defined Atom Stereocenter Count |
4
|
| SMILES |
CC1=C([C@H]([C@H]2[C@@H](C1)OC(=O)C2=C)OC(=O)C(C)C)[C@@H](C)CCCO
|
| InChi Key |
RIFCMNAQMNUNKD-ASTDHERZSA-N
|
| InChi Code |
InChI=1S/C19H28O5/c1-10(2)18(21)24-17-15(11(3)7-6-8-20)12(4)9-14-16(17)13(5)19(22)23-14/h10-11,14,16-17,20H,5-9H2,1-4H3/t11-,14+,16+,17+/m0/s1
|
| Chemical Name |
[(3aR,4S,7aR)-5-[(2S)-5-hydroxypentan-2-yl]-6-methyl-3-methylidene-2-oxo-3a,4,7,7a-tetrahydro-1-benzofuran-4-yl] 2-methylpropanoate
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.9725 mL | 14.8624 mL | 29.7247 mL | |
| 5 mM | 0.5945 mL | 2.9725 mL | 5.9449 mL | |
| 10 mM | 0.2972 mL | 1.4862 mL | 2.9725 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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