Size | Price | Stock | Qty |
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5mg |
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10mg |
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50mg |
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Other Sizes |
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Targets |
ASK1 32.8 nM (IC50)
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ln Vitro |
In AP1-HEK293 cells, ASK1-IN-2 (compound 19) (10 mM; 1 h) reduces luciferase reporter activity with a 95.59% suppression rate[1].
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ln Vivo |
In mice, dextran sulphate sodium (DSS)-induced ulcerative colitis (UC) is ameliorated by ASK1-IN-2 (25 mg/kg; po daily for 7 d)[1]. In mouse colon tissues treated with DSS, ASK1-IN-2 (25 mg/kg; po daily for 7 days) inhibits the ASK1-p38/JNK signaling pathways and lowers inflammatory cytokine levels[1]. In rats, ASK1-IN-2 (1 mg/kg; iv) has a moderate half-life (T1/2=1.45 h) and poor clearance (CL=1.38 L/h/kg[1]. Rats treated with ASK1-IN-2 (10 mg/kg; po) exhibit high oral exposure (AUClast=4517 h·ng/mL), 62.2% oral bioavailability, and a tolerable terminal half-life (T1/2=2.31 h)[1].
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Animal Protocol |
Animal/Disease Models: Male ICR mice (18 -22 g, 6-8 weeks) were given 3% DSS (w/v) orally in drinking water[1]
Doses: 25 mg/kg Route of Administration: Po daily for 7 days Experimental Results: Induced a significant recovery of body weight loss, with an increase of +11.2%. diminished the disease activity index (DAI) score about a 2 unit. Dramatically prevented colon shortening. Attenuated a severe colonic tissue damage and infiltration of inflammatory cells. Animal/Disease Models: Male SD rats [1] Doses: 1 mg/kg for iv; 10 mg/kg for po (pharmacokinetic/PK Analysis) Route of Administration: Iv and po administration Experimental Results: Iv: CL=1.38 L/h/kg; T1/2=1.45 h. Po: AUClast=4517 h• ng/mL; F=62.2%; T1/2=2.31 h. |
References |
[1]. Hou S, et, al. Structure-based discovery of 1H-indole-2-carboxamide derivatives as potent ASK1 inhibitors for potential treatment of ulcerative colitis. Eur J Med Chem. 2020 Dec 24;211:113114.
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Molecular Formula |
C19H17FN6O
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Molecular Weight |
364.38
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CAS # |
2541792-70-3
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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SMILES |
FC1C([H])=C([H])C2C([H])=C(C(N([H])C3=C([H])C([H])=C([H])C(C4=NN=C([H])N4C([H])(C([H])([H])[H])C([H])([H])[H])=N3)=O)N([H])C=2C=1[H]
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : 250 mg/mL (686.10 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (5.71 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (5.71 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 2.7444 mL | 13.7219 mL | 27.4439 mL | |
5 mM | 0.5489 mL | 2.7444 mL | 5.4888 mL | |
10 mM | 0.2744 mL | 1.3722 mL | 2.7444 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.