| Size | Price | |
|---|---|---|
| 500mg | ||
| 1g | ||
| Other Sizes |
| Targets |
LSD1
|
|---|---|
| ln Vitro |
It has been observed that arborinine (0, 5, 15, 30 µM; 48 h) increases the expression of E-cad, H3K9me1/2, and H3K4me1/2 [1]. In 786O cells, arborinine (20 µM) lowers the expression of UBE2O protein levels [1]. Twenty micrograms of arborinine (20 µM) suppresses apoptosis and causes cell cycle arrest in the S phase [1].
|
| ln Vivo |
In mice, origninine (40, 80 mg/kg; gavage; 21 days) exhibits anticancer action [2].
|
| Cell Assay |
Cell Proliferation Assay[1]
Cell Types: 786O, A498, 769P, Caki1, OSRC2 cells Tested Concentrations: 0-100 µM Incubation Duration: 48, 72 h Experimental Results: demonstrated antiproliferative activity with IC50s of 30.62, 39.09, 15.67, 31.42, 30.35 µM at 48 h, 20.92, 27.01, 14.94, 30.26, 17.37 µM at 72 h for 786O, A498, 7 69P, Caki1, OSRC2 cells, respectively. Western Blot Analysis[1] Cell Types: 786O cells Tested Concentrations: 0, 5, 15, 30 µM Incubation Duration: 48 h Experimental Results: Increased the expression of H3K4me1/2 and H3K9me1/2 protein in a dose-dependent manner. Cell Cycle Analysis[1] Cell Types: 786O and A498 cells Tested Concentrations: 20 µM Incubation Duration: Experimental Results: Induced increased population in S phase and diminished population in G1 phase, Dramatically inhibited both early and late apoptosis of ccRCC cells |
| Animal Protocol |
Animal/Disease Models: BALB/c nude mice (SGC-7901 cells and SGC-7901/ADR cells)[2]
Doses: 40, 80 mg/kg Route of Administration: Po; for 21 days Experimental Results: Inhibited the growth of tumors while unchanged the body weights. |
| References |
|
| Additional Infomation |
Arborinine is a member of acridines. It is functionally related to an acridone.
Arborinine has been reported in Almeidea coerulea, Ruta macrophylla, and other organisms with data available. |
| Molecular Formula |
C16H15NO4
|
|---|---|
| Molecular Weight |
285.2946
|
| Exact Mass |
285.1
|
| CAS # |
5489-57-6
|
| PubChem CID |
5281832
|
| Appearance |
Typically exists as solid at room temperature
|
| Density |
1.296g/cm3
|
| Boiling Point |
508.4ºC at 760 mmHg
|
| Melting Point |
175 - 176 °C
|
| Flash Point |
261.3ºC
|
| Index of Refraction |
1.621
|
| LogP |
2.414
|
| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
5
|
| Rotatable Bond Count |
2
|
| Heavy Atom Count |
21
|
| Complexity |
402
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
CN1C2=CC=CC=C2C(=O)C3=C(C(=C(C=C31)OC)OC)O
|
| InChi Key |
ATBZZQPALSPNMF-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C16H15NO4/c1-17-10-7-5-4-6-9(10)14(18)13-11(17)8-12(20-2)16(21-3)15(13)19/h4-8,19H,1-3H3
|
| Chemical Name |
1-hydroxy-2,3-dimethoxy-10-methylacridin-9-one
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.5052 mL | 17.5260 mL | 35.0521 mL | |
| 5 mM | 0.7010 mL | 3.5052 mL | 7.0104 mL | |
| 10 mM | 0.3505 mL | 1.7526 mL | 3.5052 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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