Size | Price | Stock | Qty |
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1mg |
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5mg |
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Other Sizes |
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Targets |
Bcl-2 Bax Caspase-3
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ln Vitro |
In a dose- and time-dependent way, aspidin BB (0-100 μM, 24-72 h) strongly suppressed the growth of HO-8910 cells. Following 72, 48, and 24 hours of treatment, the IC50 values were, respectively, 15.02, 25.79, and 68.81 μM[1]. In order to damage the outer mitochondrial membrane, aspidin BB inhibits Bcl-2 expression and increases Bax expression. This results in the release of cytochrome c, which activates effector caspase-3. Additionally, it cleaves poly ADP-ribose polymerase (PARP) in the nucleus, which ultimately triggers apoptosis. Demise [1]. Aspidin BB upregulates pRb, E2F1, CDK2, cyclin E, and cyclin A proteins, which results in S phase arrest in HO-8910 cells [1].
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References |
[1]. Sun Y, et al. Aspidin BB, a phloroglucinol derivative, induces cell cycle arrest and apoptosis in human ovarian HO-8910 cells. Chem Biol Interact. 2013 Jul 5;204(2):88-97.
[2]. Chen NH, et al. Drychampones A-C: Three Meroterpenoids from Dryopteris championii. J Org Chem. 2016 Oct 7;81(19):9443-9448. |
Molecular Formula |
C25H32O8
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Molecular Weight |
460.52
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CAS # |
584-28-1
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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SMILES |
CCCC(C1=C(OC)C(C)=C(O)C(CC2C(O)=C(C(CCC)=O)C(=O)C(C)(C)C=2O)=C1O)=O
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : 12.5 mg/mL (27.14 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.25 mg/mL (2.71 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1.25 mg/mL (2.71 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 2.1715 mL | 10.8573 mL | 21.7146 mL | |
5 mM | 0.4343 mL | 2.1715 mL | 4.3429 mL | |
10 mM | 0.2171 mL | 1.0857 mL | 2.1715 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.