Size | Price | Stock | Qty |
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1mg |
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5mg |
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Other Sizes |
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Targets |
Macrolide
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ln Vitro |
In a time and dose-dependent manner, bafilomycin C1 (0.33-10 μM; 6 days) suppresses the growth and proliferation of HepG2 and SMMC7721 cells[2]. In both mRNA and protein expression, bafilomycin C1 (0.33-3.3 μM; 24 hours) reduces the expression of cyclin D3, cyclin E1, CDK2, CDK4, and CDK6 in SMMC7721 cells[2]. When compared to the vehicle, bafilomycin C1 (3.3–10 μM; 24 hours) results in morphological changes and a higher number of apoptotic cells when stained with Hoechst 33258 (HY-15558)[2].
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ln Vivo |
Bafilomycin C1 (subcutaneous injection; 0.2 mg/kg; 20 days) in a model of naked mice slows the growth of tumors without causing any obvious side effects or unpleasant reactions[1].
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Cell Assay |
Cell Viability Assay[2]
Cell Types: SMMC7721 cell and HepG2 cell Tested Concentrations: 0.33 μM, 1.1 μM, and 3.3 μM for SMMC7721 1.1 μM, 3.3 μM, and 10.0 μM for HepG2 Incubation Duration: 6 days Experimental Results: Retarded the cell growth. Western Blot Analysis[2] Cell Types: SMMC7721 cells Tested Concentrations: 3.3 μM Incubation Duration: 24 hrs (hours) Experimental Results: Decreaed cyclin D3/E1,CDK2/4/6 protein expression and increased p21. Apoptosis Analysis[2] Cell Types: SMMC7721 and HepG2 cells Tested Concentrations: 3.3 μM; 10 μM Incubation Duration: 24 hrs (hours) Experimental Results: Induced apoptosis in SMMC7721 and HepG2 cells. |
Animal Protocol |
Animal/Disease Models: BALB/c nude mice (weighing 18-20 g) subcutaneous (sc)injected by SMMC7721 cell suspension (5×106 cells/100 μL)[2]
Doses: 0.2 mg/kg Route of Administration: subcutaneous (sc)injection; 20 days Experimental Results: Suppressed tumor growth of SMMC7721 tumor xenografts. |
References |
[1]. E J Bowman, et al. Bafilomycins: A Class of Inhibitors of Membrane ATPases From Microorganisms, Animal Cells, and Plant Cells. Proc Natl Acad Sci U S A. 1988 Nov;85(21):7972-6.
[2]. Xiaoxiao Gao,et al.Bafilomycin C1 Induces G0/G1 Cell-Cycle Arrest and Mitochondrial-Mediated Apoptosis in Human Hepatocellular Cancer SMMC7721 Cells. J Antibiot (Tokyo). 2018 Sep;71(9):808-817. |
Molecular Formula |
C39H60O12
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Molecular Weight |
720.89
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CAS # |
88979-61-7
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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SMILES |
OC(/C=C/C(OC1C(C)C(C(C)C)OC(C(C(C(C2OC(=O)C(OC)=CC(C)=CC(C)C(O)C(C)CC(C)=CC=CC2OC)C)O)C)(O)C1)=O)=O |c:24,t:27,37,39|
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80: Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 :Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 1.3872 mL | 6.9359 mL | 13.8717 mL | |
5 mM | 0.2774 mL | 1.3872 mL | 2.7743 mL | |
10 mM | 0.1387 mL | 0.6936 mL | 1.3872 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.