| Size | Price | Stock | Qty |
|---|---|---|---|
| 250mg |
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| Other Sizes |
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| Targets |
MIC: 20 mg/L (P. fluorescens)[1]
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|---|---|
| ln Vitro |
The percentage population of dead rat thymocyte cells varies in a dose-dependent manner in response to benzoyldodecyldimethylammonium chloride dihydrate (1–10 μM; 2 hours)[1]. With a MIC of 20 mg/L, benzoyldodecyldimethylammonium chloride dihydrate (0–40 mg/L; 0–24 hours) inhibits P. fluorescens. Cells grow in the same way as the control at 5 mg/L. The growth profile differs from the control at doses of 10 and 15 mg/L, and the cells don't begin to develop until eight hours after adaption. BDMDAC inhibits cell proliferation at doses ≥20 mg/L[2]. As evidenced by phenomena like membrane disruption and loss of membrane integrity with subsequent leakage of vital intracellular constituents, benzododecyldimethylammonium chloride dihydrate exhibits strong concentration-dependent interactions with cell surfaces and binds to microbial membrane surfaces via ionic and hydrophobic interactions[2]. These interactions promote significant and irreversible changes in the structure of the cell.
|
| Cell Assay |
Cell Viability Assay[1]
Cell Types: Rat thymocyte cells Tested Concentrations: 0, 5, 10, 15, 20 and 40 mg/L Incubation Duration: 2 hrs (hours) Experimental Results: Resulted in cell death. Cell Proliferation Assay[1] Cell Types: Bacterial P. fluorescen Tested Concentrations: 0, 5, 10, 15, 20 and 40 mg/L Incubation Duration: 2 hrs (hours) Experimental Results: Inhibited bacterial growth in a time and dose dependent manner. |
| References |
[1]. Sadegh Ghanbar, et al. New Generation of N-Chloramine/QAC Composite Biocides: Efficient Antimicrobial Agents To Target Antibiotic-Resistant Bacteria in the Presence of Organic Load. ACS Omega. 2018 Aug 22;3(8):9699-9709.
[2]. Tsuyoshi Mitani, et al. Zinc-related actions of sublethal levels of benzalkonium chloride: Potentiation of benzalkonium cytotoxicity by zinc. Chem Biol Interact. 2017 Apr 25;268:31-36. |
| Molecular Formula |
C21H42CLNO2
|
|---|---|
| Molecular Weight |
376.02
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| CAS # |
147228-80-6
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| Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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| SMILES |
[Cl-].O.O.[N+](C)(C)(CC1C=CC=CC=1)CCCCCCCCCCCC
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : 100 mg/mL (265.94 mM)
H2O : ≥ 100 mg/mL (265.94 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (6.65 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (6.65 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (6.65 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.6594 mL | 13.2972 mL | 26.5943 mL | |
| 5 mM | 0.5319 mL | 2.6594 mL | 5.3189 mL | |
| 10 mM | 0.2659 mL | 1.3297 mL | 2.6594 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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