Size | Price | Stock | Qty |
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1mg |
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5mg |
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10mg |
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50mg |
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100mg |
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Other Sizes |
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Targets |
Non-muscle myosin II (NMII)[1]
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ln Vitro |
Using bovine corneal endothelial cells, the therapeutic potential of targeting NMII to improve CEC migration was examined (BCEC). By decreasing retrograde actin flow and boosting lamellar foot process persistence, blebbistatin, a direct inhibitor of myosin motility, stimulates migration and directional persistence in CECs and speeds up in vitro wound healing [1].
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ln Vivo |
In a rabbit corneal endothelial scratch model, blebbistatin (0.05 mL, 20 μM; intracameral injection; once daily; for 6 days; New Zealand white rabbit) therapy improves wound healing [1].
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Animal Protocol |
Animal/Disease Models: New Zealand white rabbits (16-20 weeks; 3-3.5 kg)[1]
Doses: 0.05 mL; 20 μM Route of Administration: Intracameral injection; daily; for 6 days Experimental Results: Resulted in significant improvement of corneal clarity and corneal edema resolution, implying the restoration of an intact corneal endothelial monolayer. |
References |
[1]. Ho WT, et al. Targeting non-muscle myosin II promotes corneal endothelial migration through regulating lamellipodial dynamics. J Mol Med (Berl). 2019 Sep;97(9):1345-1357.
[2]. Liang Ma, et al. Discovery of the migrasome, an organelle mediating release of cytoplasmic contents during cell migration. Cell Res. 2015 Jan;25(1):24-38. |
Molecular Formula |
C18H16N2O2
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Molecular Weight |
292.33
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CAS # |
674289-55-5
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Related CAS # |
(-)-Blebbistatin;856925-71-8
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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SMILES |
O([H])C12C(C3C([H])=C(C([H])([H])[H])C([H])=C([H])C=3N=C1N(C1C([H])=C([H])C([H])=C([H])C=1[H])C([H])([H])C2([H])[H])=O
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : 25 mg/mL (85.52 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: 2.5 mg/mL (8.55 mM) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 3.4208 mL | 17.1040 mL | 34.2079 mL | |
5 mM | 0.6842 mL | 3.4208 mL | 6.8416 mL | |
10 mM | 0.3421 mL | 1.7104 mL | 3.4208 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.