| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 50mg |
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| 100mg |
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| Other Sizes |
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| ln Vitro |
Usage instructions 1. Creating the functioning DAPI solution 1.1 Getting the stock solution ready To make a 1 mg/mL stock solution, dilute 1 milligram of DAPI with 1 mL of double-distilled water. It is advised to aliquot the DAPI storage solution and keep it in the dark at -20°C or -80°C. 1.2 Making a functional solution To make a 1-10 μg/mL DAPI working solution, use either PBS dilution storage solution or hot serum-free cell culture media. Note: Before using the DAPI working fluid, please make sure that its concentration is appropriate for the current circumstances. 2. Staining of suspended cells 2.1 Centrifuge the cells to collect them, then add PBS and wash twice for five minutes each time. There are 1×106 cells per milliliter. 2.2 Add 1 milliliter of DAPI working solution, then let it sit at room temperature for three to ten minutes. 2.3 After centrifuging for three to four minutes at 400 g, remove the supernatant. 2.4 Add PBS and give the cells two 5-minute washings. 2.5 Re-suspend the cells in 1 mL of PBS or serum-free media, and use a flow cytometer or fluorescence microscope to observe. 3. Adherent cell staining 3.1 Use sterile coverslips to culture adherent cells. 3.2 Aspirate the extra culture medium after removing the coverslip from the medium. 3.3 Add 100 μL of the dye working solution, give the cells a gentle shake to cover them completely, and then let them sit for three to ten minutes. 3.4 Aspirate the dye working solution, wash with culture medium two or three times for five minutes each time, and use a flow cytometer or fluorescence microscope to monitor. Storage conditions: -20°C, one year of light protection. Notes: 1. Please modify the concentration of the DAPI working solution and the incubation period in accordance with the actual circumstances. 2. Professionals may only use this product for scientific study; it may not be utilized for clinical diagnosis or treatment, nor may it be included into food or medication. 3. Please wear a lab coat and disposable gloves for your own health and safety.
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| References |
| Molecular Formula |
C16H14BRN5
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|---|---|
| Molecular Weight |
356.219861507416
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| Exact Mass |
355.04
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| CAS # |
2387906-44-5
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| PubChem CID |
162642091
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| Appearance |
Light yellow to yellow solid powder
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| LogP |
2.3
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| Hydrogen Bond Donor Count |
5
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| Hydrogen Bond Acceptor Count |
2
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
22
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| Complexity |
446
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| Defined Atom Stereocenter Count |
0
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| SMILES |
BrC1=C(C2C=CC(C(=N)N)=CC=2)NC2C=C(C(=N)N)C=CC=21
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| InChi Key |
GSBXNZFEZFIMCS-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C16H14BrN5/c17-13-11-6-5-10(16(20)21)7-12(11)22-14(13)8-1-3-9(4-2-8)15(18)19/h1-7,22H,(H3,18,19)(H3,20,21)
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| Chemical Name |
3-bromo-2-(4-carbamimidoylphenyl)-1H-indole-6-carboximidamide
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| Synonyms |
Br-DAPI
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.8073 mL | 14.0363 mL | 28.0725 mL | |
| 5 mM | 0.5615 mL | 2.8073 mL | 5.6145 mL | |
| 10 mM | 0.2807 mL | 1.4036 mL | 2.8073 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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