| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| Other Sizes |
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| ln Vitro |
CLEFMA possesses anti-proliferative action (1-100 μM; 24-72 h)[1]. H441 and A549 cells' viability is inhibited by CLEFMA, with IC50s of 6.4 and 8.9 μM, respectively[2]. H441 and A549 cells undergo apoptosis when exposed to CLEFMA (5–10 μM; 24 h)[2]. In H441 cells, CLEFMA (1 and 10 μM; 24 h) causes autophagic death[1]. CLEFMA (1–20 μM) dose-dependently decreases NF-κB's DNA-binding activity in H441 cells[2].
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|---|---|
| ln Vivo |
In a xenograft mouse model, CLEFMA (0.2-0.4 mg/kg; ip daily for 4 weeks) suppresses the uptake of FDG in tumor tissue and inhibits tumor growth[2]. Pro-apoptotic protein BID is cleaved and pro-apoptotic BAX is expressed in tumor tissue when CLEFMA (0.2–0.4 mg/kg; intraperitoneal; daily for 4 weeks) is administered. It also downregulates the expression of anti-apoptotic markers cIAP1, Bcl-xL, Bcl-2, and survivin[2].
|
| Cell Assay |
Cell Proliferation Assay[1]
Cell Types: PANC-1, MiaPaCa -2, PC-3 and H441 cells Tested Concentrations: 1, 10, 25, 100 μM Incubation Duration: 24, 48, 72 h Experimental Results: Inhibits cell proliferation in a dose-dependent manner. |
| Animal Protocol |
Animal/Disease Models: Male athymic nu/nu (nude) mice (4 weeks old) are injected H441 cells[2]
Doses: 0.2, 0.4 mg/kg Route of Administration: Ip daily for 4 weeks Experimental Results: Inhibited tumor growth up to 96% tumor at dose of 0.4 mg/kg. |
| References |
|
| Molecular Formula |
C23H17CL2NO4
|
|---|---|
| Exact Mass |
441.053
|
| CAS # |
1246964-32-8
|
| PubChem CID |
46927962
|
| Appearance |
Typically exists as solid at room temperature
|
| LogP |
4.1
|
| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
4
|
| Rotatable Bond Count |
4
|
| Heavy Atom Count |
30
|
| Complexity |
729
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
C\1N(C/C(=C\C2=CC=CC=C2Cl)/C(=O)/C1=C/C3=CC=CC=C3Cl)C(=O)/C=C\C(=O)O
|
| InChi Key |
ABOUDPJRQGWQNW-GGDLZHBGSA-N
|
| InChi Code |
InChI=1S/C23H17Cl2NO4/c24-19-7-3-1-5-15(19)11-17-13-26(21(27)9-10-22(28)29)14-18(23(17)30)12-16-6-2-4-8-20(16)25/h1-12H,13-14H2,(H,28,29)/b10-9-,17-11+,18-12+
|
| Chemical Name |
(Z)-4-[(3E,5E)-3,5-bis[(2-chlorophenyl)methylidene]-4-oxopiperidin-1-yl]-4-oxobut-2-enoic acid
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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