| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
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| Other Sizes |
| Targets |
Carbazole-d8 itself does not have a traditional biological target; it is a stable isotope-labeled internal standard. The parent compound Carbazole is a heterocyclic aromatic compound used as a precursor in the synthesis of various pharmaceuticals and agrochemicals. Carbazole-d8 is used to track and quantify carbazole and its derivatives in biological, environmental, and pharmaceutical samples.
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|---|---|
| ln Vitro |
Drug molecules have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as tracers for quantitation during the drug development process. Due to its potential to alter the pharmacokinetic and metabolic profiles of medications, deuteration has drawn attention[1].
Carbazole-d8 does not exhibit direct in vitro biological activity as it is an analytical standard. The parent compound Carbazole is a heterocyclic aromatic compound with various applications in organic synthesis. Carbazole-d8 is used as an internal standard to accurately measure carbazole concentrations in biological and environmental samples, facilitating research on carbazole exposure and metabolism. |
| ln Vivo |
Carbazole-d8 is not intended for in vivo administration as a therapeutic agent; it is used as an analytical standard. The in vivo activity of the parent compound Carbazole is associated with its potential toxicological and pharmacological effects. Carbazole-d8 is utilized in pharmacokinetic and metabolic studies to track the distribution, metabolism, and excretion of carbazole in biological systems.
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| Enzyme Assay |
In vitro enzyme/receptor binding assays for Carbazole-d8 are not typically performed, as it is an analytical standard. Instead, the compound is used in analytical method development and validation. Quality control assays involve HPLC or LC-MS/MS to verify isotopic purity (≥98 atom % D) and chemical purity. The compound is tested for stability under various storage conditions. It appears as a solid and is soluble in organic solvents.
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| Cell Assay |
Carbazole-d8 itself is not tested in in vitro cellular assays for biological activity, as it is an analytical standard. Its utility is assessed by its performance as an internal standard in analytical workflows, including its ability to provide accurate quantification in LC-MS/MS or GC-MS analyses. The compound is used in quality control applications for carbazole analysis in environmental and pharmaceutical samples.
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| Animal Protocol |
In vivo animal studies for Carbazole-d8 are not conducted as therapeutic efficacy studies. Instead, the compound is administered to animals as a tracer in pharmacokinetic studies to quantify the parent compound carbazole in plasma, tissues, and excreta. These studies help determine the absorption, distribution, metabolism, and excretion (ADME) profile of carbazole for toxicological and regulatory purposes.
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| ADME/Pharmacokinetics |
Carbazole-d8 has a molecular formula of C12H2D8N and a molecular weight of 175.26. It has a purity of ≥98% and an isotopic enrichment of ≥98 atom % D. It appears as a solid. The compound is stable under recommended storage conditions. It is intended for research use only and is not for human consumption.
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| Toxicity/Toxicokinetics |
The toxicity profile of Carbazole-d8 is not extensively characterized, as it is an analytical standard rather than a therapeutic agent. The parent compound Carbazole has been studied for its toxicological properties. Carbazole-d8 is intended for research use only and is not approved for clinical use. Standard laboratory safety precautions should be followed when handling this compound.
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| References | |
| Additional Infomation |
Carbazole-d8 (CAS 38537-24-5) is the deuterium-labeled form of Carbazole, wherein all eight hydrogen atoms are replaced with deuterium. Carbazole is a heterocyclic aromatic compound that is used as a precursor in the synthesis of various pharmaceuticals and agrochemicals. Carbazole-d8 is used as an internal standard for the quantification of carbazole and its derivatives by GC- or LC-MS. It is intended for research use only and is not approved for clinical use.
|
| Molecular Formula |
C12HD8N
|
|---|---|
| Molecular Weight |
175.26
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| Exact Mass |
175.123
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| CAS # |
38537-24-5
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| Related CAS # |
Carbazole;86-74-8
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| PubChem CID |
59270898
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| Appearance |
Off-white to light brown solid powder
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| Density |
1.2±0.1 g/cm3
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| Boiling Point |
355.0±11.0 °C at 760 mmHg
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| Flash Point |
160.0±11.9 °C
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| Vapour Pressure |
0.0±0.8 mmHg at 25°C
|
| Index of Refraction |
1.768
|
| LogP |
3.72
|
| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
0
|
| Rotatable Bond Count |
0
|
| Heavy Atom Count |
13
|
| Complexity |
170
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| Defined Atom Stereocenter Count |
0
|
| SMILES |
[2H]C1=C(C(=C2C(=C1[2H])C3=C(C(=C(C(=C3N2)[2H])[2H])[2H])[2H])[2H])[2H]
|
| InChi Key |
UJOBWOGCFQCDNV-PGRXLJNUSA-N
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| InChi Code |
InChI=1S/C12H9N/c1-3-7-11-9(5-1)10-6-2-4-8-12(10)13-11/h1-8,13H/i1D,2D,3D,4D,5D,6D,7D,8D
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| Chemical Name |
1,2,3,4,5,6,7,8-octadeuterio-9H-carbazole
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| Synonyms |
Carbazole-d8
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~83.33 mg/mL (~475.5 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 2.08 mg/mL (11.87 mM) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.7058 mL | 28.5290 mL | 57.0581 mL | |
| 5 mM | 1.1412 mL | 5.7058 mL | 11.4116 mL | |
| 10 mM | 0.5706 mL | 2.8529 mL | 5.7058 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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