Size | Price | Stock | Qty |
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50mg |
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100mg |
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Other Sizes |
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ln Vitro |
In astrocytes, DPTIP decreases exosome release by 50% at 30 μM and inhibits EV secretion in a dose-dependent manner (0.03-30 μM) [2].
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ln Vivo |
DPTIP potently (10 mg/kg IP) inhibits IL-1β-induced astrocyte-derived EV release [1].
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Animal Protocol |
Animal/Disease Models: Mouse[1].
Doses: 10 mg/kg. Route of Administration: IL-1β was injected intraperitoneally (ip) (ip) 0.5 hrs (hrs (hours)) before striatal injection. Experimental Results: Brain concentrations of DPTIP were higher than its IC50 for nSMase2 inhibition at least 4 hrs (hrs (hours)) after compound administration. The number of astrocyte-derived EVs was diminished by 51 ± 13% 2 hrs (hrs (hours)) after IL-1β administration. |
References |
[1]. Camilo Rojas, et al. DPTIP, a newly identified potent brain penetrant neutral sphingomyelinase 2 inhibitor, regulates astrocyte-peripheral immune communication following brain inflammation. Sci Rep. 2018 Dec 7;8(1):17715.
[2]. Huarui Zhang, et al. Advances in the discovery of exosome inhibitors in cancer. J Enzyme Inhib Med Chem. 2020 Dec;35(1):1322-1330. |
Molecular Formula |
C21H18N2O3S
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Molecular Weight |
378.446
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Exact Mass |
378.1
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Elemental Analysis |
C, 66.65; H, 4.79; N, 7.40; O, 12.68; S, 8.47
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CAS # |
351353-48-5
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Appearance |
Solid powder
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SMILES |
OC1=C(OC)C=C(C2=NC(C3=CC=CC=C3)=C(C4=CC=CS4)N2)C=C1OC
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InChi Key |
JMXVHYPSBANVAQ-UHFFFAOYSA-N
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InChi Code |
InChI=1S/C21H18N2O3S/c1-25-15-11-14(12-16(26-2)20(15)24)21-22-18(13-7-4-3-5-8-13)19(23-21)17-9-6-10-27-17/h3-12,24H,1-2H3,(H,22,23)
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Chemical Name |
2,6-Dimethoxy-4-[4-phenyl-5-(2-thienyl)-1H-imidazol-2-yl]phenol
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Synonyms |
DPTIP
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : ~250 mg/mL (~660.61 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: 2.08 mg/mL (5.50 mM) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 2.6424 mL | 13.2118 mL | 26.4236 mL | |
5 mM | 0.5285 mL | 2.6424 mL | 5.2847 mL | |
10 mM | 0.2642 mL | 1.3212 mL | 2.6424 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.