Size | Price | Stock | Qty |
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10mg |
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Other Sizes |
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ln Vitro |
HCC cell growth is inhibited by demethylcantharidate (0-100 μM; 0, 12, 24, 48, or 72 hours) disodium [1]. Demethylcantharidate (0, 9, 18 or 36 μM; 24 hours) disodium raises Bax/Bcl-2, cleaved caspase-9, and cleaved caspase-3 levels in a dose-dependent manner [1].
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ln Vivo |
The in vivo hepatocellular carcinoma tumorigenesis of SMMC-7721 cells is significantly reduced by demethylcantharidate disodium [1].
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Cell Assay |
Cell Viability Assay[1]
Cell Types: HCC cell lines (SMMC-7721 and Bel-7402) Tested Concentrations: 0-100 μM Incubation Duration: 0, 12, 24, 48 or 72 hrs (hours) Experimental Results: demonstrated anti-proliferative activity in the two HCC cell lines. Cell Viability Assay[1] Cell Types: HCC cells Tested Concentrations: 0, 9, 18 or 36 µM Incubation Duration: 24 hrs (hours) Experimental Results: Induced apoptosis in HCC cells via the intrinsic pathway. |
References |
[1]. Ye M, et al. Sodium demethylcantharidate induces apoptosis in hepatocellular carcinoma cells via ER stress. Am J Transl Res. 2019;11(5):3150-3158. Published 2019 May 15.
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Molecular Formula |
C8H8NA2O5
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Molecular Weight |
230.13
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CAS # |
129-67-9
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Related CAS # |
Sodium Demethylcantharidate;13114-29-9
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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SMILES |
[Na+].[Na+].O=C(C1C2CCC(O2)C1C([O-])=O)[O-]
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
H2O : 33.33 mg/mL (144.83 mM)
DMSO : < 1 mg/mL |
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Solubility (In Vivo) |
Solubility in Formulation 1: 50 mg/mL (217.27 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
 (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 4.3454 mL | 21.7269 mL | 43.4537 mL | |
5 mM | 0.8691 mL | 4.3454 mL | 8.6907 mL | |
10 mM | 0.4345 mL | 2.1727 mL | 4.3454 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.