| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 25mg |
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| 50mg |
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| 100mg |
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| Other sizes |
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Purity: =99.4%
| Targets |
N-Myc; RKIP
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|---|---|
| ln Vitro |
Human non-small cell lung cancer cells cannot proliferate when didymin (1–20 μM) is applied for 48 hours [1]. A549 and H460 cells undergo apoptosis when exposed to didymin (10–20 μM) for 12–48 hours, but cell cycle division is unaffected [1].
|
| ln Vivo |
Didymin (6 mg/kg/day; i.p.; once daily for 28 days) delays tumor growth in nude mice [1].
|
| Enzyme Assay |
N-Myc promoter-reporter assay[2]
CHLA-90 and SMS-KCNR cells were cultured as adherent monolayers in a 96-well plate at a density of 2 × 104 cells per well. The Firefly luciferase reporter construct (0.4 ug, pEB-Luc-N-Myc), kindly gifted by Prof William L Carroll, M.D., New York University Medical Center, NY, was co-transfected with 100 ng of Renilla luciferase construct (thymidine kinase promoter-driven Renilla luciferase; pTK-RL) by Lipofectamine Reagent according to the manufacturer's instructions (Invitrogen, Carlsbad, CA). After 24 h of incubation, transfected cells were treated with 50 μM of Didymin. At 24 h post-treatment, promoter activity was measured as luciferase activity by mixing cells with 50 μl of passive lysis buffer followed by 15 min of shaking at room temperature. On a luminometer plate, 20 μl of the lysate was then mixed with 100 μl of luciferase assay reagent followed by 100 μl of Stop N' Glo Buffer. Luciferase activity was then determined as a ratio of Firefly to Renilla luciferase. Renilla luciferase activity was thus used as an internal control. |
| Cell Assay |
Cell proliferation assay [1]
Cell Types: A549 and H460 cells Tested Concentrations: 1μM, 5μM, 10μM, 20μM Incubation Duration: 48h Experimental Results: Inhibition of cell proliferation of human non-small cell lung cancer cells. Apoptosis analysis [1] Cell Types: A549 and H460 cells Tested Concentrations: 10 μM, 20 μM Incubation Duration: 12 h, 24 h, 48 h Experimental Results: Induced apoptosis of A549 and H460 cells without affecting cell cycle distribution. |
| Animal Protocol |
Animal/Disease Models: Male nude mouse [6 weeks old; 28 days old]. BALB/cA-nu (nu/nu)] injection of A549 cells [1]
Doses: 6 mg/kg/day Route of Administration: intraperitoneal (ip) injection; one time/day for 28 days Experimental Results: Inhibited tumor growth in nude mice. |
| References |
[1]. Hung JY, et al. Didymin, a dietary flavonoid glycoside from citrus fruits, induces Fas-mediated apoptotic pathway in human non-small-cell lung cancer cells in vitro and in vivo. Lung Cancer. 2010 Jun;68(3):366-74.
[2]. Singhal J, et al. Didymin induces apoptosis by inhibiting N-Myc and upregulating RKIP in neuroblastoma. Cancer Prev Res (Phila). 2012 Mar;5(3):473-83. |
| Molecular Formula |
C28H34O14
|
|---|---|
| Molecular Weight |
594.5612
|
| Exact Mass |
594.19488
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| CAS # |
14259-47-3
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| Appearance |
White to yellow solid
|
| LogP |
2.72
|
| tPSA |
214.06
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| SMILES |
O1[C@]([H])([C@@]([H])([C@]([H])([C@@]([H])([C@@]1([H])C([H])([H])O[C@@]1([H])[C@@]([H])([C@@]([H])([C@]([H])([C@]([H])(C([H])([H])[H])O1)O[H])O[H])O[H])O[H])O[H])O[H])OC1=C([H])C(=C2C(C([H])([H])[C@@]([H])(C3C([H])=C([H])C(=C([H])C=3[H])OC([H])([H])[H])OC2=C1[H])=O)O[H]
|
| InChi Key |
RMCRQBAILCLJGU-HIBKWJPLSA-N
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| InChi Code |
InChI=1S/C28H34O14/c1-11-21(31)23(33)25(35)27(39-11)38-10-19-22(32)24(34)26(36)28(42-19)40-14-7-15(29)20-16(30)9-17(41-18(20)8-14)12-3-5-13(37-2)6-4-12/h3-8,11,17,19,21-29,31-36H,9-10H2,1-2H3/t11-,17-,19+,21-,22+,23+,24-,25+,26+,27+,28+/m0/s1
|
| Chemical Name |
(2S)-5-hydroxy-2-(4-methoxyphenyl)-7-[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-[[(2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methyloxan-2-yl]oxymethyl]oxan-2-yl]oxy-2,3-dihydrochromen-4-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~250 mg/mL (~420.48 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.17 mg/mL (3.65 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 21.7 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.17 mg/mL (3.65 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 21.7 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.17 mg/mL (3.65 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.6819 mL | 8.4096 mL | 16.8192 mL | |
| 5 mM | 0.3364 mL | 1.6819 mL | 3.3638 mL | |
| 10 mM | 0.1682 mL | 0.8410 mL | 1.6819 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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