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100mg | ||
250mg | ||
500mg | ||
Other Sizes |
ln Vitro |
At a dose of 12.5 μM, G43 (16 h) exhibits an 85% inhibitory rate against Streptococcus mutans biofilm [1]. G43 (25 μM, 24 h) inhibits glycosyltransferase (Gtfs) glucan synthesis and constantly suppresses GtfB and GtfC activities, with an 80% inhibition rate for both enzymes [1]. In UA159 mutans Streptococcus mutans, G43 (up to 25 μM, 24 h) has no discernible effect on the expression of gtfs (gtfB, gtfC, and gtfD) [1]. G43 (50 μM, 24-48 h) inhibits the formation of biofilms in wild-type Streptococcus mutans by decreasing the synthesis of water-insoluble exopolysaccharides [2].
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ln Vivo |
G43 (100 µM) used topically twice a day for four weeks decreases Streptococcus mutans' pathogenicity in vivo [1].
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Cell Assay |
RT-PCR[1]
Cell Types: S. mutans. UA159 cells Tested Concentrations: 3.125, 6.25, 12, 25 μM Incubation Duration: 24 h Experimental Results: demonstrated no significant difference on expression of gtfs (gtfB, gtfC and gtfD). |
Animal Protocol |
Animal/Disease Models: The rat model of dental caries infected with S. mutans UA159[1]
Doses: 100 µM twice (two times) daily for 4 weeks Route of Administration: Topically administration Experimental Results: diminished the buccal, sulcal, and proximal surface caries scores, but did not lose weight over the course of the study. |
References |
[1]. Zhang Q, et al. Structure-Based Discovery of Small Molecule Inhibitors of Cariogenic Virulence. Sci Rep. 2017;7(1):5974.
[2]. Scaffa PMC, et al. The potential use of glycosyl-transferase inhibitors for targeted reduction of S. mutans biofilms in dental materials. Sci Rep. 2023;13(1):11889. |
Molecular Formula |
C16H11N3O4S
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Molecular Weight |
341.34
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CAS # |
690693-02-8
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80: Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 :Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 2.9296 mL | 14.6482 mL | 29.2963 mL | |
5 mM | 0.5859 mL | 2.9296 mL | 5.8593 mL | |
10 mM | 0.2930 mL | 1.4648 mL | 2.9296 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.