| Size | Price | |
|---|---|---|
| 500mg | ||
| 1g | ||
| Other Sizes |
| ln Vitro |
Insulin secretion in MIN6 cells was considerably increased by HWL-088 (0.3 μM and 3 μM) at a concentration of 25 mM, but not at a glucose concentration of 2 mM. With 25 mM glucose present, HWL-088 exhibits dose-dependent insulinotropic effects [2].
|
|---|---|
| ln Vivo |
HWL-088 (40 mg/kg; oral gavage; daily; for 30 days; ob/ob mice) therapy enhances beta-cell activity and lowers fat formation in adipose tissue through overexpression of pancreaticoduodenal homeobox-1 , alleviates fatty liver disease in ob/ob mice. The benefits of HWL-088 include lowering hepatic lipogenesis and oxidative stress, boosting lipoprotein lipolysis, glucose absorption, mitochondrial activity, and fatty acid beta-oxidation [2].
|
| Animal Protocol |
Animal/Disease Models: Male ob/ob mice [2]
Doses: 40 mg/kg Route of Administration: po (oral gavage); daily; continued for 30 days Experimental Results: Improved β-cell function through upregulation of pancreaticoduodenal homeobox-1 , reduce fat accumulation in adipose tissue and alleviate fatty liver disease in ob/ob mice. |
| References |
|
| Molecular Formula |
C22H19FO4
|
|---|---|
| Molecular Weight |
366.382
|
| Exact Mass |
366.126
|
| CAS # |
2378617-96-8
|
| PubChem CID |
139600331
|
| Appearance |
White to off-white solid powder
|
| Density |
1.2±0.1 g/cm3
|
| Boiling Point |
523.7±45.0 °C at 760 mmHg
|
| Flash Point |
270.5±28.7 °C
|
| Vapour Pressure |
0.0±1.4 mmHg at 25°C
|
| Index of Refraction |
1.591
|
| LogP |
5.23
|
| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
5
|
| Rotatable Bond Count |
7
|
| Heavy Atom Count |
27
|
| Complexity |
471
|
| Defined Atom Stereocenter Count |
0
|
| InChi Key |
NJYJBYPNDWNZAH-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C22H19FO4/c1-15-5-2-3-8-19(15)17-7-4-6-16(11-17)13-26-18-9-10-21(20(23)12-18)27-14-22(24)25/h2-12H,13-14H2,1H3,(H,24,25)
|
| Chemical Name |
2-[2-fluoro-4-[[3-(2-methylphenyl)phenyl]methoxy]phenoxy]acetic acid
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~250 mg/mL (~682.35 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 2.08 mg/mL (5.68 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (5.68 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.08 mg/mL (5.68 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.7294 mL | 13.6470 mL | 27.2941 mL | |
| 5 mM | 0.5459 mL | 2.7294 mL | 5.4588 mL | |
| 10 mM | 0.2729 mL | 1.3647 mL | 2.7294 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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