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| 100mg | ||
| 250mg | ||
| 500mg | ||
| Other Sizes |
| ln Vitro |
In AW1 cells, isochamaejasmin (6.25-100μM; 24-72 h) demonstrated possible toxicity in a dose- and time-dependent way. Time-varying potential against H is exhibited by isochamaejasmin (1000 mg/L, 500 mg/L, 250 mg/L, 125 mg/L, 62.5 mg/L; 24 h, 48 h, 72 h, and 96 h). The toxicity and dose-dependent behavior of zea larvae [1]. In AW1 cells, isochamaejasmin (40-80μM; 24 h) damages DNA and raises γH2AX and OGG1 levels. In the G2/M phase, stop the cell cycle [1]. A dose-dependent mechanism of apoptosis induction in AW1 cells is induced by isochamaejasmin (20-80μM; 24 h)[1]. Isochamaejasmin (20-80μM; 24 h) exhibits a dose-dependent increase in reactive oxygen species (ROS) levels, accumulation of lipid peroxidation products, and inactivation of antioxidant enzymes in AW1 cells. It also shows a decrease in MMP and an upregulation of Bax/Bcl-2 expression, which causes the release of cytochrome c into the cytosol, activation of caspase-3/9, and PARP cleavage [1]. With an EC50 of 3.23 μM, isochamaejasmin stimulates the expression of a reporter gene controlled by NF-κB in transfected HeLa cells. A dominant-negative construct of IκBα blocks isochamaejasmin-stimulated NF-κB reporter activity, which is followed by nuclear translocation of NF-κB protein. Together with PKCδ, isochamaejasmin also phosphorylates ERK1/2, p38, and other mitogen-activated protein kinases in a time-dependent manner [2].
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| Cell Assay |
Cell Viability Assay[1]
Cell Types: Larvae and neuronal cells (AW1) Tested Concentrations: 6.25 μM, 12.5 μM, 25 μM, 50 μM and 100 μM Incubation Duration: 24 h, 48 h and 72 h Experimental Results: Had potential toxicity against H. zea both in vivo and in vitro via time- and dose-dependent manners. Cell Cycle Analysis[1] Cell Types: Neuronal cells ( AW1) Tested Concentrations: 40 μM and 80 μM Incubation Duration: 24 h Experimental Results: The cell cycle was arrested at the G2/M phase. Apoptosis Analysis[1] Cell Types: Neuronal cells (AW1) Tested Concentrations: 20 μM, 40 μM, and 80 μM Incubation Duration: 24 h Experimental Results: Induced apoptosis via the mitochondrial pathway in AW1 cells. Western Blot Analysis[1] Cell Types: Neuronal cells (AW1) Tested Concentrations: 20 μM, 40 μM, and 80 μM Incubation Duration: 24 h Experimental Results: demonstrated upregulation of Bax/Bcl-2 expression resulting in the release of cytochrome c into the cytosol, activation of caspase-3/9, and cleavage of PARP. |
| References |
[1]. Yuanhang Ren, et al. Isochamaejasmin induces toxic effects on Helicoverpa zea via DNA damage and mitochondria-associated apoptosis. Pest Manag Sci. 2021 Jan;77(1):557-567.
[2]. Qinghai Tian, et al. Stereospecific induction of nuclear factor-kappaB activation by isochamaejasmin. Mol Pharmacol. 2005 Dec;68(6):1534-42. [3]. Liene Dhooghe, et al. Antiplasmodial activity of (I-3,II-3)-biflavonoids and other constituents from Ormocarpum kirkii. Phytochemistry. 2010 May;71(7):785-91. |
| Molecular Formula |
C30H22O10
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|---|---|
| Molecular Weight |
542.49
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| CAS # |
93859-63-3
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| Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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| SMILES |
O=C1C2C(=CC(=CC=2O[C@@H](C2C=CC(O)=CC=2)[C@@H]1[C@H]1C(=O)C2C(=CC(=CC=2O[C@@H]1C1C=CC(O)=CC=1)O)O)O)O
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.8434 mL | 9.2168 mL | 18.4335 mL | |
| 5 mM | 0.3687 mL | 1.8434 mL | 3.6867 mL | |
| 10 mM | 0.1843 mL | 0.9217 mL | 1.8434 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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