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100mg | ||
250mg | ||
500mg |
ln Vitro |
Together with Runx2, KY-02327 (1–10 μM; 2 days; MC3T3E1 cells, a mouse preosteoblast cell line) elevates β-catenin protein levels and dose-dependently accumulates nuclear β-catenin [1]. The osteoblast development markers collagen 1a (Col1a) and osteocalcin (OCN) have their mRNA levels increased by KY-02327 (1-10 μM) [1].
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ln Vivo |
KY-02327 (20 mg/kg; oral; 5 days per week for 4 weeks) successfully recovered bone loss in an ovariectomized (OVX) mice model [1].
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Animal Protocol |
Animal/Disease Models: 8weeks old female BL6 mice (ovariectomy (OVX)-induced osteoporosis model mice) [1]
Doses: 20 mg/kg Route of Administration: Po; administered 5 days a week for 4 days Experimental Results: Newly formed bone labeled with calcein was diminished in the femurs of vehicle-treated OVX mice. |
References |
[1]. Kim HY, et al. Small molecule inhibitors of the Dishevelled-CXXC5 interaction are new drug candidates for bone anabolic osteoporosis therapy. EMBO Mol Med. 2016;8(4):375-387.
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Molecular Formula |
C20H27N3O4
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Molecular Weight |
373.453
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CAS # |
2093407-25-9
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Related CAS # |
KY-02327 acetate
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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SMILES |
O=C(CN1C(C(=O)OCC)=CC2C=C(C=CC1=2)O)NCCN1CCCCC1
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : ~100 mg/mL (~267.77 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 4.24 mg/mL (11.35 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 42.4 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (6.69 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (6.69 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 2.6777 mL | 13.3887 mL | 26.7773 mL | |
5 mM | 0.5355 mL | 2.6777 mL | 5.3555 mL | |
10 mM | 0.2678 mL | 1.3389 mL | 2.6777 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.