Size | Price | Stock | Qty |
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5mg |
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10mg |
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Other Sizes |
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ln Vitro |
The minimum inhibitory concentration (MIC) of lactoferrin 17-41 (lacoferrin B) acetate against Escherichia coli ATCC 25922 is 30 μg/mL [1]. Lactoferrin 17–41 acetate exhibits cytotoxic action on HT-29 cells and dramatically increases HT-29 cell apoptosis [2]. To varied degrees, lactoferrin 17–41 acetate can control the transcription of genes such PMAIP-1, TP5313, and SFN that are implicated in the p53 signaling pathway[2]. Human monocyte LPS-induced cytokine responses can be inhibited by lactoferrin 17-41 acetate, which can bind LPS from Gram-negative bacteria [1][3].
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Cell Assay |
Cell Viability Assay[1]
Cell Types: HT-29 cells Tested Concentrations: 50, 100, 200, 400, 800 or 1000 µg/mL Incubation Duration: 4, 12, 24 or 48 hrs (hours) Experimental Results: More effective at inducing apoptosis at 400 µg/mL. Higher toxicity is shown at 800 µg/mL. |
References |
[1]. Samuelsen Ø, et al. Anti-complement effects of lactoferrin-derived peptides. FEMS Immunol Med Microbiol. 2004 Jun 1;41(2):141-8.
[2]. Jiang R, et al. Bovine lactoferrin and lactoferricin exert antitumor activities on human colorectal cancer cells(HT-29) by activating various signaling pathways. Biochem Cell Biol. 2017 Feb;95(1):99-109. [3]. Latorre D, et al. Reciprocal interactions between lactoferrin and bacterial endotoxins and their role in the regulation of the immune response. Toxins (Basel). 2010;2(1):54‐68. |
Molecular Formula |
C143H226N46O33S3
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Molecular Weight |
3183.82
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CAS # |
2828433-30-1
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Related CAS # |
Lactoferrin (17-41);146897-68-9
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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SMILES |
C(=O)(O)C.C([C@@H]1NC(=O)C(NC([C@@]([H])(NC([C@H](CSSC[C@]([H])(NC([C@@]([H])(NC([C@@]([H])(NC([C@@H](NC([C@]2([H])CCCN2C(=O)[C@H](C)NC(=O)CNC(=O)[C@]([H])(CC(C)C)NC(=O)[C@H](CCCCN)NC(=O)[C@]([H])(CCCCN)NC(=O)[C@H](CCSC)NC(=O)[C@H](CCCNC(N)=N)NC(=O)C(CC2=CNC3=CC=CC=C23)NC(=O)[C@H](CCC(=O)N)NC1=O)=O)CO)=O)[C@@H](C)CC)=O)[C@H](O)C)=O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(=O)N[C@H](C(=O)O)CC1C=CC=CC=1)NC(=O)[C@H](CCCCN)NC(=O)[C@@H](N)CC1C=CC=CC=1)=O)CCCNC(N)=N)=O)CCCNC(N)=N)C1=CNC2=CC=CC=C12
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : 20 mg/mL (6.28 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2 mg/mL (0.63 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2 mg/mL (0.63 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2 mg/mL (0.63 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 0.3141 mL | 1.5704 mL | 3.1409 mL | |
5 mM | 0.0628 mL | 0.3141 mL | 0.6282 mL | |
10 mM | 0.0314 mL | 0.1570 mL | 0.3141 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.