Size | Price | Stock | Qty |
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5mg |
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10mg |
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50mg |
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100mg |
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Other Sizes |
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ln Vitro |
In vitro, microtubule polymerization is stimulated by MAP4343 (40 μM; 15 or 30 min)[1]. In PC12 cells, MAP4343 (30 μM; 2–8 d) increases neurite outgrowth induced by nerve growth factor (NGF)[1]. In PC12 cells, MAP4343 (20 μM; 1 h) guards against microtubule depolymerization brought on by nocodazole[1].
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ln Vivo |
Rats with spinal cord injury (SCI) recover more quickly after taking MAP4343 (12 mg/kg; sc daily for 28 days)[2]. After SCI in rats, MAP4343 (4 mg/kg; daily SC for 6 days) raises MAP2 levels in the spinal cord[2]. After SCI in rats, MAP4343 (4 mg/kg; daily sc for 6 d) increases the size of the dendritic arbours of lumbar spinal motoneurons[2].
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Animal Protocol |
Animal/Disease Models: Adult SD (Sprague-Dawley) male rats with spinal cord injury[2]
Doses: 12 mg/kg Route of Administration: Daily sc for 28 days Experimental Results: Increased the number of animals able to walk with weight-supported plantar steps. demonstrated forelimb-hindlimb coordination at the end of the study. |
References |
[1]. Fontaine-Lenoir V, et, al. Microtubule-associated protein 2 (MAP2) is a neurosteroid receptor. Proc Natl Acad Sci U S A. 2006 Mar 21;103(12):4711-6.
[2]. Duchossoy Y, et, al. Treatment of experimental spinal cord injury with 3β-methoxy-pregnenolone. Brain Res. 2011 Jul 27;1403:57-66. |
Molecular Formula |
C22H34O2
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Molecular Weight |
330.50
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CAS # |
511-26-2
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Related CAS # |
MAP4343-d4
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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SMILES |
COC1CCC2(C3CCC4(C(CCC4C3CC=C2C1)C(=O)C)C)C
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : 25 mg/mL (75.64 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: 2.5 mg/mL (7.56 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (7.56 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 3.0257 mL | 15.1286 mL | 30.2572 mL | |
5 mM | 0.6051 mL | 3.0257 mL | 6.0514 mL | |
10 mM | 0.3026 mL | 1.5129 mL | 3.0257 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.