Size | Price | Stock | Qty |
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5mg |
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10mg |
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50mg |
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100mg |
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Other Sizes |
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Targets |
β-barrel assembly machine A (BamA)[1]
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ln Vitro |
MRL-494 causes the cytoplasmic membrane to rupture fatally. By focusing on BamA, MRL-494 prevents the synthesis of OM proteins (OMPs) from outside the outer membrane (OM). Strong anti-microbial effects against both Gram-positive and Gram-negative bacteria are demonstrated by MRL-494. The MRL-494 MIC values against E. Coli (WT), E. Coli (ΔtolC), E. K. Coli (\tolC envA101). pneumonia, A. baumannii (WT), A. Baumannii (ΔlpxC), Pseudomonas aeruginosa (efflux deficient), Pseudomonas aeruginosa (WT), Methicillin-resistant Staphylococcus aureus, and Bacillus subtilis rpoB18 have respective concentrations of 25 μM, 25 μM, 25 μM, 100 μM, 200 μM, 100 μM, 12.5 μM, and 25 μM[1].
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References |
[1]. Hart EM, A small-molecule inhibitor of BamA impervious to efflux and the outer membrane permeability barrier. Proc Natl Acad Sci U S A. 2019 Oct 22;116(43):21748-21757.
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Molecular Formula |
C26H36CLFN16O2
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Molecular Weight |
659.12
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CAS # |
2699937-04-5
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Related CAS # |
MRL-494;2434898-43-6
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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SMILES |
N1C(=NN(CC(=O)N[C@H]2CC[C@@H](NC3=NC(NC(C4CC4)CC(NC(=N)N)=O)=NC(NC(N)=N)=N3)CC2)N=1)C1C=CC(F)=CC=1.Cl
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage. (2). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
H2O : 110 mg/mL (166.89 mM)
DMSO : 100 mg/mL (151.72 mM) |
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Solubility (In Vivo) |
Solubility in Formulation 1: 25 mg/mL (37.93 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with heating and sonication.
 (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 1.5172 mL | 7.5859 mL | 15.1717 mL | |
5 mM | 0.3034 mL | 1.5172 mL | 3.0343 mL | |
10 mM | 0.1517 mL | 0.7586 mL | 1.5172 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.