| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
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| 500mg |
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| Other Sizes |
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| Targets |
Plasmodium
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|---|---|
| ln Vitro |
Methylene blue functions as a substitute electron acceptor/donor, which helps to repair mitochondria, enhances neural energy generation, and prevents superoxide from forming[1]. Cytochrome P450 (CYP) isozymes are inhibited by methylene blue. When combined with water, methylene blue, an odorless, water-soluble, dark blue-green crystalline powder, turns blue. A vasopressor called methylene blue inhibits inducible NOS, which in turn inhibits the activation of sGC, which is how it affects the NO synthesis pathway. Methylene blue also inhibits the buildup of cyclic GMP (cGMP) by attaching to the iron heme moiety of sGC and directly competing with NO for the ability to activate soluble guanylyl cyclase[3].
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| ln Vivo |
In male Sprague-Dawley rats (7-week-old, 200-250 g), methylene blue (1, 5, and 25 μg/rat) dramatically reduces brain cyclic guanosine monophosphate (cGMP) content and sevoflurane minimum alveolar anesthetic concentration (MAC) in a dose-dependent manner[2]. Methylene blue is sprayed into the gastrointestinal tract mucosa during chromoendoscopy as a dye to detect dysplasia, or precancerous lesions[2]. Mean arterial pressures (MAP) can be normalized, vascular tone can be restored, and the need for vasopressors can be decreased with methylene blue[3].
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| References |
[1]. Delport A, et al. Methylene blue and its analogues as antidepressant compounds. Metab Brain Dis. 2017 Oct;32(5):1357-1382.
[2]. Masaki E, et al. Methylene blue, a soluble guanylyl cyclase inhibitor, reduces the sevoflurane minimum alveolar anesthetic concentration and decreases the brain cyclic guanosine monophosphate content in rats. Anesth Analg. 1999 Aug;89(2):484-9. [3]. McCartney SL, et al. Intraoperative vasoplegia: methylene blue to the rescue! Curr Opin Anaesthesiol. 2018 Feb;31(1):43-49. |
| Molecular Formula |
C16H24CLN3O3S
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|---|---|
| Molecular Weight |
373.90
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| CAS # |
7220-79-3
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| Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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| SMILES |
[Cl-].S1C2C([H])=C(C([H])=C([H])C=2N=C2C([H])=C([H])/C(/C([H])=C12)=[N+](/C([H])([H])[H])\C([H])([H])[H])N(C([H])([H])[H])C([H])([H])[H].O([H])[H].O([H])[H].O([H])[H]
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : 100 mg/mL (267.45 mM)
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|---|---|
| Solubility (In Vivo) |
10% DMSO+ 40% PEG300+ 5% Tween-80+ 45% saline : ≥ 2.5 mg/mL (6.69 mM) (Please use freshly prepared in vivo formulations for optimal results.)
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| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.6745 mL | 13.3726 mL | 26.7451 mL | |
| 5 mM | 0.5349 mL | 2.6745 mL | 5.3490 mL | |
| 10 mM | 0.2675 mL | 1.3373 mL | 2.6745 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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