| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
|
||
| 10mg |
|
||
| 50mg |
|
||
| 100mg |
|
||
| Other Sizes |
|
| ln Vitro |
Millepachine (1.25–20 μM; 48 h) significantly reduces the growth of A2780CP cells that are resistant to cisplatin[1]. Apoptosis and G2/M arrest are induced in cisplatin-sensitive A2780S and cisplatin-resistant A2780CP cells by millepachine (2–8 μM) within 24 or 48 hours[1]. In A2780S and A2780CP cells, millepachine (2–8 μM; 24 h) reduces topoisomerase II levels[1]. In A2780CP cells, millepachine (2–8 μM; 24 h) suppresses the activity of the ATP-binding cassette transporter [1].
|
|---|---|
| ln Vivo |
Mice exposed to millepachine (20 mg/kg; intravenously every two days for 14 days) do not develop tumors[1]. In an excised A2780S xenograft model, millepachine (20 mg/kg; iv every two days for 14 days) did not result in acquired drug resistance[1].
|
| Cell Assay |
Cell Proliferation Assay[1]
Cell Types: A2780CP cells Tested Concentrations: 0, 1.25, 2.5, 5, 10, 20 μM Incubation Duration: 48 hrs (hours) Experimental Results: Inhibited the cells proliferation with an IC50 of 4 μM. Cell Cycle Analysis[1] Cell Types: A2780S and A2780CP cells Tested Concentrations: 2, 4, 8 μM Incubation Duration: 24 or 48 hrs (hours) Experimental Results: Induced significant G2/M arrest both in both cells. The percentage of cells in the G2/M fraction increased from 15.99% in vehicle cells to 24.93%, 60.67%, and 77.31% at dose of 2, 4, and 8 μM in A2780S cells, . Apoptosis Analysis[1] Cell Types: A2780S and A2780CP cells Tested Concentrations: 2, 4, 8 μM Incubation Duration: 24 or 48 hrs (hours) Experimental Results: The percentage of apoptotic cells increased from 1.49% in vehicle cells to 10.98%, 20.60%, respectively. and 39.43% at dose of 2, 4, and 8 μM in A2780S cells, respectively. The percentage of apoptotic cells increased from 0.87% to 10.97%, 25.28%, and 37.59% in A2780CP cells, respectively. Western Blot Analysis[1] Cell Types: A2780S and A2780CP cells Concen |
| Animal Protocol |
Animal/Disease Models: Female BALB/c nude mice (6 weeks) are injected A2780S or A2780CP cells[1]
Doses: 20 mg/kg Route of Administration: Iv every two days for 2-14 days Experimental Results: diminished tumor volume and tumor weight with the inhibitory rate of 73.21% and 65.58% in A2780S (after seven times injection) and A2780CP (after six times injection) xenograft model, respectively. With low toxicity in vivo. |
| References |
[1]. Wu W, et, al. Millepachine showed novel antitumor effects in cisplatin-resistant human ovarian cancer through inhibiting drug efflux function of ATP-binding cassette transporters. Phytother Res. 2018 Dec; 32(12):2428-2435.
|
| Molecular Formula |
C22H22O4
|
|---|---|
| Molecular Weight |
350.41
|
| CAS # |
1393922-01-4
|
| Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
|
| SMILES |
O1C2C(C(/C=C/C3C=CC(=CC=3)OC)=O)=CC=C(C=2C=CC1(C)C)OC
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : 100 mg/mL (285.38 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 2.5 mg/mL (7.13 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (7.13 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.8538 mL | 14.2690 mL | 28.5380 mL | |
| 5 mM | 0.5708 mL | 2.8538 mL | 5.7076 mL | |
| 10 mM | 0.2854 mL | 1.4269 mL | 2.8538 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
