Size | Price | Stock | Qty |
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1mg |
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5mg |
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10mg |
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Other Sizes |
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ln Vitro |
Compound 1, O,O-Dimethyl-cannabigerol, inhibits the development of KB cells with IC50 values of 69.4 μM (SRB assay) and 44.55 μM (MTT assay)[2]. When administered to NIH 3T3 fibroblasts, O,O-Dimethyl-cannabigerol (0-100 μM) over 48 hours causes cell cytotoxicity, with CD50s of 60.46 μM (MTT assay) and 82.98 μM (SRB assay)[2]. O,O-Dimethyl-cannabigerol has an IC50 of 31.3 μM and exhibits antitumor efficaciousness against mouse skin melanoma cells[2].
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Cell Assay |
Cell Cytotoxicity Assay[2]
Cell Types: NIH 3T3 fibroblasts Tested Concentrations: 0, 1, 25, 50, 100 μM Incubation Duration: 48 hrs (hours) Experimental Results: Induced cell cytotoxic with CD50s of 60.46 μM (MTT assay) and 82.98 μM (SRB assay) |
References |
[1]. Appendino G, et al. Antibacterial cannabinoids from Cannabis sativa: a structure-activity study. J Nat Prod. 2008;71(8):1427-1430.
[2]. Han DS, et al. Synthesis and cytotoxic effects of deoxy-tomentellin. Arch Pharm Res. 2000;23(2):121-127. [3]. Caprioglio D, et al. O-Methyl Phytocannabinoids: Semi-synthesis, Analysis in Cannabis Flowerheads, and Biological Activity. Planta Med. 2019;85(11-12):981-986. |
Molecular Formula |
C23H36O2
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Molecular Weight |
344.53
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CAS # |
29106-16-9
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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SMILES |
CCCCCC1=CC(OC)=C(C/C=C(\CC/C=C(\C)/C)/C)C(OC)=C1
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : 55 mg/mL (159.64 mM)
H2O : < 0.1 mg/mL |
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Solubility (In Vivo) |
Solubility in Formulation 1: 2.75 mg/mL (7.98 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 27.5 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 2.75 mg/mL (7.98 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 27.5 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.75 mg/mL (7.98 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 2.9025 mL | 14.5125 mL | 29.0250 mL | |
5 mM | 0.5805 mL | 2.9025 mL | 5.8050 mL | |
10 mM | 0.2903 mL | 1.4513 mL | 2.9025 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.