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100mg | ||
250mg | ||
500mg | ||
Other Sizes |
ln Vitro |
For A549 cells, oenothein B (15-45 µM; 12-72 hours) exhibits a dose- and time-dependent inhibitory rate effect at exposure times of 12, 24, 36, 48, 60, and 72 hours [1]. By causing apoptosis and stopping cells at the G1 stage, oenothein B (15–45 µM; 24 hours) efficiently suppresses the growth of A549 cells[1]. In addition to elevating intracellular reactive oxygen species (ROS) levels, oenothein B (15–45 µM; 24 hours) also causes the elevation of intracellular apoptotic triggers, such as cleavage caspase-3, PARP, cytochrome c level in the cytoplasm, and Bax[1]. cells A549[1] within 24 hours at 15 µM, 30 µM, and 45 µM cells in the G1 phase that have been arrested. cells A549[1] within 24 hours at 15 µM, 30 µM, and 45 µM considerably downregulated were PARP, p53, cytochrome c (cytoplasm), and BAX; anti-apoptotic Bcl-2 was considerably reduced in a concentration-dependent manner.
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ln Vivo |
In ICR mice undergoing systemic inflammation, oenothein B (100–300 mg/kg; po) can lessen neuroinflammation in the brain[3].
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Cell Assay |
Cell Viability Assay[1]
Cell Types: A549 cells Tested Concentrations: 15 µM, 30 µM and 45 µM Incubation Duration: 12 hrs (hours), 24 hrs (hours), 36 hrs (hours), 48 hrs (hours), 60 hrs (hours) and 72 hrs (hours) Experimental Results: Effectively inhibited the proliferation of A549 cells. Apoptosis Analysis[1] Cell Types: A549 cells Tested Concentrations: 15 µM, 30 µM and 45 µM Incubation Duration: 24 hrs (hours) Experimental Results: Induced apoptosis in A549 cells. Cell Cycle Analysis[1] Cell Types: A549 cells Tested Concentrations: 15 µM, 30 µM and 45 µM Incubation Duration: 24 hrs (hours) Experimental Results: Arrested cells in the G1 phase. Western Blot Analysis[1] Cell Types: A549 cells Tested Concentrations: 15 µM, 30 µM and 45 µM Incubation Duration: 24 hrs (hours) Experimental Results: BAX, p53, cytochrome c (cytoplasm) and PARP were unregulated Dramatically; Anti-apoptotic Bcl-2 was diminished Dramatically in a concentration-dependent manner. |
References |
[1]. Xiaodong Pei, et al. Oenothein B inhibits human non-small cell lung cancer A549 cell proliferation by ROS-mediated PI3K/Akt/NF-κB signaling pathway. Chem Biol Interact. 2019 Jan 25;298:112-120.
[2]. Satoru Tamura, et al. Oenothein B, dimeric hydrolysable tannin inhibiting HCV invasion from Oenothera erythrosepala. J Nat Med. 2019 Jan;73(1):67-75. [3]. Satoshi Okuyama, et al. Oenothein B suppresses lipopolysaccharide (LPS)-induced inflammation in the mouse brain. Int J Mol Sci. 2013 May 7;14(5):9767-78. |
Molecular Formula |
C68H50O44
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Molecular Weight |
1571.10
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CAS # |
104987-36-2
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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SMILES |
O1[C@]([H])([C@@]([H])([C@]([H])([C@@]2([H])[C@@]1([H])C([H])([H])OC(C1=C([H])C(=C(C(=C1C1=C(C(=C(C([H])=C1C(=O)O2)O[H])O[H])O[H])O[H])O[H])O[H])=O)OC(C1C([H])=C(C(=C(C=1[H])O[H])O[H])O[H])=O)OC(C1=C([H])C(=C(C(=C1OC1=C(C(=C2C(=C1[H])C(=O)OC([H])([H])[C@]1([H])[C@@]([H])([C@]([H])([C@]([H])([C@]([H])(OC(C3C([H])=C(C(=C(C=3[H])O[H])O[H])O[H])=O)O1)O[H])OC(C1C([H])=C(C(=C(C=1[H])O[H])O[H])O[H])=O)OC(C1=C([H])C(=C(C(=C12)O[H])O[H])O[H])=O)O[H])O[H])O[H])O[H])O[H])=O)O[H]
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80: Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 :Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 0.6365 mL | 3.1825 mL | 6.3650 mL | |
5 mM | 0.1273 mL | 0.6365 mL | 1.2730 mL | |
10 mM | 0.0636 mL | 0.3182 mL | 0.6365 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.