| Size | Price | Stock | Qty |
|---|---|---|---|
| 1g |
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| 5g |
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| Other Sizes |
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| Targets |
IL-4 IL-13 TNFR1
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| ln Vivo |
Induction of colitis [1] 1. Method of preparation: Vortex acetone and olive oil at a 4:1 v/v ratio to create the sensitizing solution (1). To create a 3% (w/v) oxazolone-sensitized solution, dissolve 60 mg of oxazolone powder in 2 mL of this mixture. Use a gentle vortex to blend the mixture. Challenge solution (2) preparation: To make a 1% (w/v) solution, dissolve 20 mg of oxazolone in 2 mL of 50% ethanol. Use a gentle vortex to blend the mixture. Before used, the powdered oxazolone needs to be completely dissolved. 2. Procedure for the experiment: Sensitization pretreatment: Using electric scissors, scrape off around 2 cm by 2 cm of the mouse's back skin. Take care not to get any open wounds. Mice's naked skin should be given 150 μL of Oxazolone Sensitizing Solution (I). Take note that the control was a sensitizer free of oxazolone. Treatment induction: Give mice an intraperitoneal injection of ketamine/thiazide solution (80 μL/10 g) or use an isoflurane anesthesia device to anesthetize them by inhalation. Place the catheter in the mouse's colon, about 3–4 cm deep, right next to the anus. Attach the catheter's opposite end to a 1 mL syringe. Using the catheter, inject around 100 μL of Oxazolone Stimulating Solution (II) into the colon lumen for ten to thirty seconds. The mouse was kept vertically (head down) for 60 seconds following treatment.
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| References |
[1]. Weigmann B, et al. Oxazolone-Induced Colitis as a Model of Th2 Immune Responses in the Intestinal Mucosa. Methods Mol Biol. 2016;1422:253-61.
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| Molecular Formula |
C12H11NO3
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|---|---|
| Molecular Weight |
217.22
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| CAS # |
15646-46-5
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| Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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| SMILES |
O=C(OC(C1=CC=CC=C1)=N/2)C2=C/OCC
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : 100 mg/mL (460.36 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (11.51 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.6036 mL | 23.0181 mL | 46.0363 mL | |
| 5 mM | 0.9207 mL | 4.6036 mL | 9.2073 mL | |
| 10 mM | 0.4604 mL | 2.3018 mL | 4.6036 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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