Size | Price | Stock | Qty |
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5mg |
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10mg |
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50mg |
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Other Sizes |
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ln Vitro |
POMHEX (78 nM, 8h) significantly affects ENO1-deficient cells but has no impact on ENO1-WT glioma cells [1]. POMHEX (0-720 nM) specifically causes energy stress, suppresses growth, and initiates death in glioma cells lacking ENO1 [1].
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ln Vivo |
Hemolytic anemia was consistently avoided with POMHEX (iv, ip) injections at doses up to 10 mg/kg (body weight) per day. POMHEX (intravenous, 35 mg/kg) makes patients drowsy, which leads vets to start the euthanasia process [1]. In isolated mouse plasma, POMHEX hydrolyzes quickly to Hemi POMHEX, which has a half-life of roughly 30 seconds, while in isolated human blood, it has a half-life of almost 9 minutes [1].
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Cell Assay |
Cell Proliferation Assay[1]
Cell Types: ENO1-deleted (D423, red), ENO1-isogenically rescued (D423 ENO1, blue) and ENO1-WT (LN319, gray) cells. Tested Concentrations: 78 nM. Incubation Duration: 8 h. Experimental Results: Down-regulated cell density. |
References |
[1]. Yu-Hsi Lin, et al. An enolase inhibitor for the targeted treatment of ENO1-deleted cancers. Nat Metab. 2020 Nov 23.
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Molecular Formula |
C17H30NO9P
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Molecular Weight |
423.40
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CAS # |
2004714-34-3
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Related CAS # |
Hex;2004714-32-1
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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SMILES |
C1CCN(O)C(C1P(=O)(OCOC(C(C)(C)C)=O)OCOC(O)C(C)(C)C)=O
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : 100 mg/mL (236.18 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (5.90 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (5.90 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (5.90 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 2.3618 mL | 11.8092 mL | 23.6183 mL | |
5 mM | 0.4724 mL | 2.3618 mL | 4.7237 mL | |
10 mM | 0.2362 mL | 1.1809 mL | 2.3618 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.