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5mg | ||
Other Sizes |
ln Vitro |
Patritumab binds to the extracellular domain (ECD) of HER3 and causes DiFi-HRG4 cells to undergo apoptosis (10 μg/mL; 5 d)[1]. In DiFi-HRG4 cells, patriotumab (10 μg/mL; 6 h) significantly reduces HER3 and AKT phosphorylation while leaving ERK phosphorylation unaffected[1]. In addition to inducing the cleavage of PARP, patriotumab (10 μg/mL; 48 h) also up-regulates BIM and down-regulates survivin expression[1].
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ln Vivo |
In the DiFi-HRG tumor xenografts model in mice, patritumab (1 mg/mouse; ip; twice weekly for 4 weeks) interacts with 1 mg Cetuximab to restore Cetuximab sensitivity[1]. Cetuximab resistance is induced by heregulin, which is produced by colorectal cancer tumors expressing wild-type KRAS. Cetuximab resistance is overcome in vivo by combination therapy involving cetuximab and patritumab[1].
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Cell Assay |
Western Blot Analysis[1]
Cell Types: DiFi-HRG cells Tested Concentrations: 10 μg/mL Incubation Duration: 6 hrs (hours) Experimental Results: Inhibited the phosphorylation of HER3 and AKT as well as down-regulated survivin expression. |
Animal Protocol |
Animal/Disease Models: Female athymic nude mice (BALB/c; 5 -6 weeks old) with DiFi-Mock1 or DiFi-HRG4 (sc)[1]
Doses: 1 mg/body Route of Administration: intraperitoneal (ip)injection; twice a week for 4 weeks Experimental Results: Individual Patritumab treatment had little effect on the growth of tumors formed by either cell line. Combination of Cetuximab and Patritumab induced substantial regression of DiFi-HRG4 xenografts. |
References |
[1]. Kawakami H, et al. The anti-HER3 antibody patritumab abrogates cetuximab resistance mediated by heregulin in colorectal cancer cells. Oncotarget. 2014 Dec 15;5(23):11847-56.
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CAS # |
1262787-83-6
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80: Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 :Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400  (Please use freshly prepared in vivo formulations for optimal results.) |
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.