Size | Price | Stock | Qty |
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5mg |
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10mg |
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50mg |
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100mg |
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Other Sizes |
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ln Vitro |
Pitstop 2 (20–40 μM; 30 min) prevents J774A.1 macrophages from endocytosing transferrin while maintaining cell viability[1]. Pitstop 2 (20–40 μM; 30 min) has no effect on J774A.1 macrophages' ability to internalize cholera toxin B[1]. Pitstop 2 (0.001-100 μM; 6 h) inhibits the spindle of HeLa cells, preventing them from progressing through mitosis[2]. Pitstop 2 (1-30 μM; 24 h) inhibits the growth of dividing cancer cells and causes apoptosis[2]. Pitstop 2 (1-30 μM; 48 h) has no effect on non-tumourigenic NIH3T3 fibroblasts' ability to proliferate and remain viable[2].
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Cell Assay |
Cell Viability Assay[2]
Cell Types: HeLa cells Tested Concentrations: 1, 3, 10, 30 μM Incubation Duration: 24 hrs (hours) Experimental Results: decreased the total number of viable HeLa cells markedly in a dose-dependent manner. |
References |
[1]. Kumar GA, et, al. Leishmania donovani Internalizes into Host Cells via Caveolin-mediated Endocytosis. Sci Rep. 2019 Sep 2; 9(1): 12636.
[2]. Smith CM, et, al. Inhibition of clathrin by pitstop 2 activates the spindle assembly checkpoint and induces cell death in dividing HeLa cancer cells. Mol Cancer. 2013 Jan 17; 12:4. |
Molecular Formula |
C20H13BRN2O3S2
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Molecular Weight |
473.36
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CAS # |
1419320-73-2
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Related CAS # |
Clathrin-IN-1;1332879-52-3
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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SMILES |
C1(C=CC=C2C=CC=CC=12)S(=O)(=O)NC1S/C(=C\C2C=CC(Br)=CC=2)/C(=O)N=1
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : 62.5 mg/mL (132.03 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (5.28 mM) (saturation unknown) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 2.1126 mL | 10.5628 mL | 21.1256 mL | |
5 mM | 0.4225 mL | 2.1126 mL | 4.2251 mL | |
10 mM | 0.2113 mL | 1.0563 mL | 2.1126 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.