| Size | Price | |
|---|---|---|
| 500mg | ||
| 1g | ||
| Other Sizes |
| ln Vitro |
Compound 8 (SJ1008030) (72 hours) exhibits activity in the MHH-CALL-4 CRLF2r cell line, with an IC50 of 5.4 nM[1]. JAK-STAT signaling nutrients of JAK, GSPT1, and IKZF1 in a dosed manner is suggested by the dose-dependent degradation of JAK2 and GSPT1 proteins in xenografted bone marrow cells SJBALL021415 by SJ1008030 (0-4.3 μM; 72 hours) in MHH-CALL-4 cells and SJ1008030 (0-10 μM; 24 hours)[1].
|
|---|---|
| Cell Assay |
Cytotoxicity assay[1]
Cell Types: CRLF2r cell lines MHH–CALL-4, KOPN49, MHH–CALL-2, KOP N75, NALM-6 and 697 Tested Concentrations: 0-3 μM Incubation Duration: 72 Experimental Results: In MHH–CALL The best activity was shown in -4 cells with an EC50 value of 5.4 nM. Western Blot Analysis[1] Cell Types: MHH–CALL-4 Cells Tested Concentrations: 1 nM, 10 nM, 100 nM, 1 μM, and 4.3 μM Incubation Duration: 72 hrs (hours) Experimental Results: Significant JAK2-dependent degradation in MHH–CALL-4 cells sex, while the effect on JAK1, JAK3 and GSPT1 is weak. Western Blot Analysis [1] Cell Types: Xenograft SJBALL021415 Bone Marrow Cell Tested Concentrations: 1, 10, 100, 1000 and 1000 nM Incubation Duration: 24 hrs (hours) Experimental Results: JAK2 was nearly completely degraded in a dose-dependent manner, with no effect on GSPT1. |
| References |
| Molecular Formula |
C42H43N13O7S
|
|---|---|
| CAS # |
2863634-96-0
|
| Related CAS # |
SJ1008030 TFA;SJ1008030 formic
|
| Appearance |
Typically exists as solid at room temperature
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
