Size | Price | Stock | Qty |
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1mg |
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5mg |
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10mg |
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Other Sizes |
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ln Vitro |
In senescent cells, SSK1 (0.5 μM; 12-72 hours) stimulates the phosphorylation levels of MKK3/MKK6 and p38 MAPK. Senescent cells are eliminated by SSK1 through the p38 MAPK signaling pathway. In senescent cells, SSK1 can cause damage to the mitochondrial DNA [1]. SSK1 (0.01-1 μM; 3 days) effectively and selectively removes β-galactosidase-positive senescent cells within a wide processing window [1].
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ln Vivo |
In rats with lung damage, SSK1 (0.5 mg/kg; intraperitoneal injection; two days per week; for four weeks) lowers aging-related indicators and removes senescent cells [1]. SSK1 (0.5 mg/kg; 3 days every 2 weeks for 8 weeks) attenuates low-grade local and systemic inflammation, successfully clears senescent cells in various organs, and decreases senescence and age-related genetic markers in mice (20 months of age) [1].
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Cell Assay |
Cell Proliferation Assay[1]
Cell Types: Primary mouse fibroblasts Tested Concentrations: 0.5 µM Incubation Duration: 12 hrs (hours), 24 hrs (hours), 36 hrs (hours), 48 hrs (hours), 72 hrs (hours) Experimental Results: Both p38 MAPK and MKK3/MKK6 were activated by phosphorylation in senescent cells. |
Animal Protocol |
Animal/Disease Models: Mice (3-6-month-old) were subjected to transtracheal injection of Bleomycin[1]
Doses: 0.5 mg/kg Route of Administration: intraperitoneally (ip) injection ; two days every week; for four weeks Experimental Results: SSK1 Dramatically diminished the percentage of SA-β-gal-positive cells in lung by 3.8-fold compared with that in vehicle-treated lung-injured mice |
References |
[1]. Yusheng Cai, et al. Elimination of senescent cells by β-galactosidase-targeted prodrug attenuates inflammation and restores physical function in aged mice. Cell Res. 2020 Jul;30(7):574-589.
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Molecular Formula |
C31H34F2N4O18
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Molecular Weight |
788.61
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CAS # |
2629250-69-5
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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SMILES |
O(C1C=CC(COC(=O)NC2C=CN([C@H]3C([C@H](O)[C@@H](CO)O3)(F)F)C(=O)N=2)=CC=1N(=O)=O)[C@@H]1O[C@H](COC(=O)C)[C@H](OC(=O)C)[C@H](OC(=O)C)[C@H]1OC(=O)C
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : 200 mg/mL (253.61 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 5 mg/mL (6.34 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 50.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 5 mg/mL (6.34 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 50.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 1.2681 mL | 6.3403 mL | 12.6805 mL | |
5 mM | 0.2536 mL | 1.2681 mL | 2.5361 mL | |
10 mM | 0.1268 mL | 0.6340 mL | 1.2681 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.