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1mg |
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Other Sizes |
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ln Vitro |
(-)-Sculerine, with IC50s ranging from 2.7 µM to 6.5 µM, suppresses a mini-panel of human leukemic cells, including MOLT-4 (WT), Raji (TP53 mutant), Jurkat (TP53 mutated), HL-60 (TP53 null), U-937 (TP53 mutated), and HEL 92.1.7 (wild-type)[1]. The growth of Jurkat and MOLT-4 cells is inhibited by scopulerine (2.5–20 µM; 24 hours)[1]. MOLT-4 and Jurkat cells undergo apoptosis when exposed to (2.5–20 µM; 24 hours)[1]. Induced G2 or M cell cycle arrest is caused by scopulerine [1]. p53 protein is upregulated in p53 wild-type MOLT-4 cells when scoulerine (2.5–5 µM) is applied for 24 hours[1]. Activation of caspase-3/7, -8, and -9 is dose-dependent and occurs at 2.5–5 µM over a 24-48-hour period[1]. The microtubule structure of A549 lung cancer cells is disrupted by scopulerine (5–10 µM) over a 24-hour period[1].
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Cell Assay |
Cell Proliferation Assay[1]
Cell Types: Jurkat and MOLT -4 cells Tested Concentrations: 2.5, 5, 10, 15 and 20 µM Incubation Duration: 24 hrs (hours) Experimental Results: Dramatically decreased the viability and proliferation of Jurkat and MOLT-4 cells in a dose dependent manner. Apoptosis Analysis[1] Cell Types: MOLT -4 and Jurkat cells Tested Concentrations: 2.5, 5, 10, 15 and 20 µM Incubation Duration: 24 hrs (hours) Experimental Results: Induced MOLT-4 and Jurkat cells apoptosis. Cell Cycle Analysis[1] Cell Types: Jurkat and MOLT-4 leukemic cells Tested Concentrations: 2.5-20 µM Incubation Duration: 16 hrs (hours) Experimental Results: Induced cell cycle arrest at the G2/M transition. Western Blot Analysis[1] Cell Types: MOLT-4 cells Tested Concentrations: 2.5, 5 µM Incubation Duration: 24 hrs (hours) Experimental Results: demonstrated an upregulation of p53 protein in p53 wild-type MOLT-4 cells. |
References |
[1]. Habartova K, et al. Scoulerine affects microtubule structure, inhibits proliferation, arrests cell cycle and thus culminates in the apoptotic death of cancer cells. Sci Rep. 2018;8(1):4829. Published 2018 Mar 19.
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Molecular Formula |
C19H21NO4
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Molecular Weight |
327.37
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CAS # |
6451-73-6
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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SMILES |
O([H])C1=C(C([H])=C([H])C2=C1C([H])([H])N1C([H])([H])C([H])([H])C3=C([H])C(=C(C([H])=C3[C@]1([H])C2([H])[H])O[H])OC([H])([H])[H])OC([H])([H])[H]
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : 100 mg/mL (305.46 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (6.35 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (6.35 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 3.0546 mL | 15.2732 mL | 30.5465 mL | |
5 mM | 0.6109 mL | 3.0546 mL | 6.1093 mL | |
10 mM | 0.3055 mL | 1.5273 mL | 3.0546 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.