| Size | Price | |
|---|---|---|
| 500mg | ||
| 1g | ||
| Other Sizes |
| ln Vitro |
Treatment with TAK-960 monohydrochloride results in an accumulation of G2-M cells, abnormal polo mitotic morphology, and elevated histone H3 phosphorylation (pHH3). With mean EC50 values ranging from 8.4 to 46.9 nM, TAK-960 monohydrochloride (2-1000 nM; 72 hours) suppresses the growth of various cancer cell lines, but not nondividing normal cells[1]. In HeLa cells, TAK-960 monohydrochloride (8 nM) causes G2/M cell cycle arrest without causing appreciable cytotoxicity[2].
|
|---|---|
| ln Vivo |
TAK-960 monohydrochloride shows notable activity against various tumor xenografts (10 mg/kg; po; once daily for 2 weeks)[1]. TAK-960 monohydrochloride (po) strongly suppresses the growth of HT-29 colorectal cancer xenografts in animal models and raises pHH3 in a dose-dependent manner[1].
|
| Cell Assay |
Cell Viability Assay[1]
Cell Types: HT-29, HCT116, COLO320DM, HCT-15, RKO, SW480, K-562…. Hela, DU 145 cells Tested Concentrations: 2-1000 nM Incubation Duration: 72 hrs (hours) Experimental Results: Inhibited proliferation of human cancer cell lines regardless of TP53 and KRAS mutation and MDR1 expression status. |
| Animal Protocol |
Animal/Disease Models: nude mice or SCID (severe combined immunodeficient) mouse (bearing HCT116, PC-3, BT474, A549 , NCI-H1299, NCI-H1975, A2780, and MV4-11 cells)[1]
Doses: 10 mg/kg Route of Administration: Po; one time/day for 2 weeks Experimental Results: Substantial antitumor activity and good tolerability. |
| References |
|
| Molecular Formula |
C27H35CLF3N7O3
|
|---|---|
| Molecular Weight |
598.06
|
| Exact Mass |
597.244
|
| CAS # |
2108449-45-0
|
| Related CAS # |
TAK-960;1137868-52-0;TAK-960 dihydrochloride;TAK-960 hydrochloride;1137868-96-2
|
| PubChem CID |
91826087
|
| Appearance |
Typically exists as solid at room temperature
|
| Hydrogen Bond Donor Count |
3
|
| Hydrogen Bond Acceptor Count |
11
|
| Rotatable Bond Count |
6
|
| Heavy Atom Count |
41
|
| Complexity |
903
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
Cl.FC1(C(N(C)C2C=NC(NC3C=C(C(=CC=3OC)C(NC3CCN(C)CC3)=O)F)=NC=2N(C1)C1CCCC1)=O)F
|
| InChi Key |
QZBHDFGFNVMONB-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C27H34F3N7O3.ClH/c1-35-10-8-16(9-11-35)32-24(38)18-12-22(40-3)20(13-19(18)28)33-26-31-14-21-23(34-26)37(17-6-4-5-7-17)15-27(29,30)25(39)36(21)2;/h12-14,16-17H,4-11,15H2,1-3H3,(H,32,38)(H,31,33,34);1H
|
| Chemical Name |
4-[(9-cyclopentyl-7,7-difluoro-5-methyl-6-oxo-8H-pyrimido[4,5-b][1,4]diazepin-2-yl)amino]-2-fluoro-5-methoxy-N-(1-methylpiperidin-4-yl)benzamide;hydrochloride
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.6721 mL | 8.3604 mL | 16.7207 mL | |
| 5 mM | 0.3344 mL | 1.6721 mL | 3.3441 mL | |
| 10 mM | 0.1672 mL | 0.8360 mL | 1.6721 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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