Size | Price | Stock | Qty |
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1mg |
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5mg |
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10mg |
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Other Sizes |
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Targets |
Staphylococcal nuclease and tudor domain containing 1, SND1; [3,5-(2)H(2)] Tyrosyl nuclease[1][2]
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ln Vitro |
The hepatocytes of WT and Alb/SND1 (which express SND1 transgenic micro p65 expression levels and p65 nuclear translocation in mice) are considerably reduced by thymidine 3',5'-diphosphate tetrasodium (200 µM; 18 h). Sphere formation in WT and Alb/SND1 hepatocytes is inhibited by thymidine 3',5'-diphosphate tetrasodium [1]
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ln Vivo |
There is no discernible effect of thymidine 3',5'-diphosphate tetrasodium (0.8 mg/kg; intraperitoneal injection; twice weekly for 4 weeks) on blood liver enzymes (AST, ALT, AP), total protein (TP) in WT B6/CBA mice, albumin (Alb), and globulin (Glo) [1]. In WT B6/CBA mice, thymidine 3',5'-diphosphate tetrasodium (0.8 mg/kg and 1.6 mg/kg; intravenous injection; twice weekly for 4 weeks) effectively reduces tumor growth [1]. In adult male NSG mice, the expression of thymidine 3',5'-diphosphate tetrasodium (0.8, 0.16, and 0.32 mg/kg; subcutaneous injection; twice weekly for 4 weeks) suppresses tumor proliferation, inflammatory response, and tumor originating cell (TIC) signatures. The expression of PTEN, TGFBR2, and CDKN1C apoptosis as well as specific tumor suppressor genes are up-regulated by thymidine 3',5'-diphosphate tetrasodium [1].
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Animal Protocol |
Animal/Disease Models: 2 months old WT B6/CBA mice[1].
Doses: 0.8 mg/kg. Route of Administration: intraperitoneal (ip)injection; twice a week for 4 weeks . Experimental Results: Had insignificant effect on serum liver enzymes, total protein, albumin and globulin and demonstrated biosafety. Animal/Disease Models: NSG mice[1]. Doses: 0.8, 0.16 or 0.32 mg/kg. Route of Administration: intravenous (iv)injection or subcutaneous (sc)injection; twice a week for 4 weeks. Experimental Results: Inhibited tumor growth, demonstrated antitumor activity and immunomodulatory effects. |
References |
[1]. Nidhi Jariwala, et al. Oncogenic Role of SND1 in Development and Progression of Hepatocellular Carcinoma. Cancer Res. 2017 Jun 15;77(12):3306-3316.
[2]. Cohen J S, et al. Proton magnetic resonance studies of the tyrosine residues of staphylococcal nuclease using [3, 5-2H2] tyrosine[J]. Biochimica et Biophysica Acta (BBA)-Protein Structure, 1971, 236(2): 468-478. |
Molecular Formula |
C10H12N2NA4O11P2
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Molecular Weight |
490.12
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CAS # |
118675-87-9
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Related CAS # |
Thymidine 3',5'-disphosphate;2863-04-9
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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SMILES |
O=C1NC(=O)C(C)=CN1[C@H]1C[C@H](OP(O)(O)=O)[C@@H](COP(O)(O)=O)O1.[NaH]
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
H2O : 50 mg/mL (102.02 mM)
DMSO : 3.33 mg/mL (6.79 mM) |
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Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80: Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 :Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 2.0403 mL | 10.2016 mL | 20.4032 mL | |
5 mM | 0.4081 mL | 2.0403 mL | 4.0806 mL | |
10 mM | 0.2040 mL | 1.0202 mL | 2.0403 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.