| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| 5mg |
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| 10mg |
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| Other Sizes |
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| Targets |
IC50: 280 nM (METTL3)[1]
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|---|---|
| ln Vitro |
UZH1a (2.5-160 μM; 72 h) suppresses the development of U2Os, HEK293T, and MOLM-13 cells with IC50 values of 11 μM, 67 μM, and 87 μM, respectively [1]. UZH1a (2.5–100 μM; 16 h) dose-dependently lowers the m6A methylation levels in MOLM-13 cell mRNA (IC50= 4.6 μM) [1]. UZH1a (40 μM; 16 hours) decreases the amounts of m6A methylation in the mRNA of MOLM-13, HEK293T, and U2Os cells [1]. MOLM-13 cells experience cell cycle arrest and increased apoptosis when exposed to UZH1a (20 μM) for 16 hours [1].
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| Cell Assay |
Cell Viability Assay[1]
Cell Types: MOLM-13, HEK293T, and U2Os cells Tested Concentrations: 2.5-160 µM Incubation Duration: 72 hrs (hours) Experimental Results: Inhibited the growth of MOLM-13, HEK293T, and U2Os cells in a dose-dependent manner. |
| References |
| Molecular Formula |
C32H42N6O3
|
|---|---|
| Molecular Weight |
558.714287281036
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| Exact Mass |
558.331
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| CAS # |
2813577-78-3
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| Related CAS # |
UZH1;2925713-02-4;UZH1b;2814392-17-9
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| PubChem CID |
154815692
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| Appearance |
Off-white to light yellow solid powder
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| LogP |
4.8
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
8
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| Rotatable Bond Count |
9
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| Heavy Atom Count |
41
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| Complexity |
828
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| Defined Atom Stereocenter Count |
1
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| SMILES |
CC1(CCN(CC1)CC2=CC(=C(C=C2)C(=O)NC[C@@]3(CCCN(C3)C4=NC=NC(=C4)NCC5=CC=CC=C5)O)O)C
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| InChi Key |
PWYRDVXYAOGDNK-JGCGQSQUSA-N
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| InChi Code |
InChI=1S/C32H42N6O3/c1-31(2)12-15-37(16-13-31)20-25-9-10-26(27(39)17-25)30(40)34-21-32(41)11-6-14-38(22-32)29-18-28(35-23-36-29)33-19-24-7-4-3-5-8-24/h3-5,7-10,17-18,23,39,41H,6,11-16,19-22H2,1-2H3,(H,34,40)(H,33,35,36)/t32-/m1/s1
|
| Chemical Name |
N-[[(3R)-1-[6-(benzylamino)pyrimidin-4-yl]-3-hydroxypiperidin-3-yl]methyl]-4-[(4,4-dimethylpiperidin-1-yl)methyl]-2-hydroxybenzamide
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : 80 mg/mL (143.19 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 6 mg/mL (10.74 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 60.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. Solubility in Formulation 2: ≥ 5.5 mg/mL (9.84 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 55.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 5.5 mg/mL (9.84 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.7898 mL | 8.9492 mL | 17.8984 mL | |
| 5 mM | 0.3580 mL | 1.7898 mL | 3.5797 mL | |
| 10 mM | 0.1790 mL | 0.8949 mL | 1.7898 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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