| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| Other Sizes |
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| ln Vitro |
Xylopine exhibits strong cytotoxicity in a dose- and time-dependent manner (3.5 μM–14 μM; 24-48 hours)[1]. With IC50 values ranging from 6.4 to 26.6 μM in eight distinct cancer cell lines (MCF7, HCT116, HepG2, SCC-9, HSC-3, HL-60, K-562, and B16-F10), xylopine (72 h) has cytotoxic activity[1]. Internucleosomal DNA fragmentation follows cell cycle arrest at phase G2/M caused by xylopine (3.5 μM–14 μM; 24-48 hours)[1]. 3.5 μM–14 μM; 24-48 hours) of xylopine significantly enhances caspase-3 activation, produces mitochondrial depolarization, and increases early and late apoptosis[1]. 1]. G2/M phase arrest was induced in HCT116 cells[1] at 3.5 μM, 7 μM, and 14 μM for 24 and 48 hours. In HCT116 cells[1], the early and late apoptosis was significantly increased by 3.5 μM, 7 μM, and 14 μM after 24 and 48 hours.
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| Cell Assay |
Cell Viability Assay[1]
Cell Types: HCT116 cells Tested Concentrations: 3.5 μM, 7 μM, and 14 μM Incubation Duration: 24 hrs (hours), 48 hrs (hours) Experimental Results: Displayed potent cytotoxicity in HCT116 cells. Cell Cycle Analysis[1] Cell Types: HCT116 cells Tested Concentrations: 3.5 μM, 7 μM, and 14 μM Incubation Duration: 24 hrs (hours), 48 hrs (hours) Experimental Results: Induced G2 /M phase arrest. Apoptosis Analysis[1] Cell Types: HCT116 cells Tested Concentrations: 3.5 μM, 7 μM, and 14 μM Incubation Duration: 24 hrs (hours), 48 hrs (hours) Experimental Results: Dramatically increased the early and late apoptosis. |
| References | |
| Additional Infomation |
Xylopine is an aporphine alkaloid.
Xylopine has been reported in Magnolia liliifera, Xylopia parviflora, and other organisms with data available. |
| Molecular Formula |
C18H17NO3
|
|---|---|
| Molecular Weight |
295.33
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| Exact Mass |
295.121
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| CAS # |
517-71-5
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| PubChem CID |
160503
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| Appearance |
Light yellow to yellow solid powder
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| Density |
1.289g/cm3
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| Boiling Point |
483.4ºC at 760mmHg
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| Flash Point |
193.7ºC
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| Index of Refraction |
1.635
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| LogP |
3.162
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
22
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| Complexity |
431
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| Defined Atom Stereocenter Count |
1
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| SMILES |
COC1=CC2=C(C=C1)C3=C4[C@@H](C2)NCCC4=CC5=C3OCO5
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| InChi Key |
RFWCCZDSXIZJMF-CQSZACIVSA-N
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| InChi Code |
InChI=1S/C18H17NO3/c1-20-12-2-3-13-11(6-12)7-14-16-10(4-5-19-14)8-15-18(17(13)16)22-9-21-15/h2-3,6,8,14,19H,4-5,7,9H2,1H3/t14-/m1/s1
|
| Chemical Name |
(12R)-16-methoxy-3,5-dioxa-11-azapentacyclo[10.7.1.02,6.08,20.014,19]icosa-1(20),2(6),7,14(19),15,17-hexaene
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.3860 mL | 16.9302 mL | 33.8604 mL | |
| 5 mM | 0.6772 mL | 3.3860 mL | 6.7721 mL | |
| 10 mM | 0.3386 mL | 1.6930 mL | 3.3860 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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