Size | Price | Stock | Qty |
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10mg |
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25mg |
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50mg |
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100mg |
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250mg |
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500mg |
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Other Sizes |
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Acacetin is a novel and potent Flavonoid that is derived from Dendranthema morifolium. Acacetin causes cell cycle arrest, apoptosis, and autophagy in cancer cells by docking in the ATP-binding pocket of PI3Kγ . Acacetin can be used for research on diseases associated with pain because it has potential anti-inflammatory and anti-cancer activity.
ln Vitro |
Acacetin (5,7-Dihydroxy-4'-methoxyflavone; 10-200 μM; 24 hours) decreases cell viabilities in a dose-dependent manner.
Human normal glial cell line HEB and non-tumorigenic epithelial cell line MCF-10A are not significantly affected by acetin[1]. Acacetin (50-150 μM; 24 hours) induces apoptosis and autophagy and causes G2/M cell cycle arrest[1]. Acacetin (50-150 μM; 24 hours) causes dose-dependent reductions in the levels of PI3Kγ-p110, p-AKT, p-mTOR, p-p70S6K and p-ULK. |
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ln Vivo |
Acacetin (5,7-Dihydroxy-4'-methoxyflavone; 5, 20 mg/kg/day; orally; for 3 days) significantly suppresses microglial activation in an LPS-induced neuroinflammation mouse model[2].
Acacetin (25 mg/kg/day; orally; for 3 days) lowers neuronal cell death in an animal model of ischemia[2]. Acacetin (1.8-56.2 mg/kg/day; intraperitoneal; single dose) reduces visceral and inflammatory nociception and prevents the formalin-induced oedema[3]. |
Animal Protocol |
Male C57BL/6 mice, 7 weeks of age[2]
5, 20 mg/kg Orally; once a day for 3 days |
References |
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Molecular Formula |
C16H12O5
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Molecular Weight |
284.267
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Exact Mass |
284.0685
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Elemental Analysis |
C, 67.60; H, 4.26; O, 28.14
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CAS # |
480-44-4
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Related CAS # |
480-44-4
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Appearance |
Solid powder
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SMILES |
COC1=CC=C(C=C1)C2=CC(=O)C3=C(C=C(C=C3O2)O)O
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InChi Key |
DANYIYRPLHHOCZ-UHFFFAOYSA-N
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InChi Code |
InChI=1S/C16H12O5/c1-20-11-4-2-9(3-5-11)14-8-13(19)16-12(18)6-10(17)7-15(16)21-14/h2-8,17-18H,1H3
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Chemical Name |
5,7-dihydroxy-2-(4-methoxyphenyl)chromen-4-one
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Synonyms |
NSC 76061; Acacetin
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO: ~125 mg/mL (~439.7 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (7.32 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. Solubility in Formulation 2: 5 mg/mL (17.59 mM) in 50% PEG300 50% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. View More
Solubility in Formulation 3: 10 mg/mL (35.18 mM) in 0.5% CMC-Na/saline water (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. Solubility in Formulation 4: 2.5 mg/mL (8.79 mM) in 0.5% CMC/saline water (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 3.5178 mL | 17.5889 mL | 35.1778 mL | |
5 mM | 0.7036 mL | 3.5178 mL | 7.0356 mL | |
10 mM | 0.3518 mL | 1.7589 mL | 3.5178 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.