ACBI1

Cat No.:V41715 Purity: ≥98%
ACBI1 (ACBI-1) is PROTAC degrader of BAF ATPase subunits SMARCA2 and SMARCA4 (DC50 = 6 nM, 11 nM and 32 nM for SMARCA2, SMARCA4 and PBRM1 in MV-4-11 cells) with anticancer activity.
ACBI1 Chemical Structure CAS No.: 2375564-55-7
Product category: PROTACs
This product is for research use only, not for human use. We do not sell to patients.
Size Price Stock Qty
5mg
10mg
25mg
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Product Description

ACBI1 (ACBI-1) is PROTAC degrader of BAF ATPase subunits SMARCA2 and SMARCA4 (DC50 = 6 nM, 11 nM and 32 nM for SMARCA2, SMARCA4 and PBRM1 in MV-4-11 cells) with anticancer activity.It is composed of a bromodomain ligand, a linker, and the E3 ubiquitin ligase VHL. ACBI1 has anti-proliferative effects and can induceapoptosis. Italso degrades the polybromo-associated BAF (PBAF) complex member PBRM1.

Biological Activity I Assay Protocols (From Reference)
ln Vitro
ACBI1 exhibits anti-proliferative action at 1–10,000 nM over 3–7 days [1]. SK-MEL-5 cells undergo apoptosis when exposed to ACBI1 (0.3 µM; 100 hours) [1]. The E3 ubiquitin ligase von Hippel-Lindau, a linker, and a bromodomain ligand make up ACBI1[1].
Cell Assay
Cell proliferation assay[1]
Cell Types: MV-4-11, NCI-H1568 Cell
Tested Concentrations: 0-1000 nM
Incubation Duration: 3-7 days
Experimental Results: demonstrated anti-proliferative activity, IC50 of MV-4 is 29, 68 nM -11, NCI-H1568 cells.

Apoptosis analysis[1]
Cell Types: SK-MEL-5 Cell
Tested Concentrations: 0.3 µM
Incubation Duration: 100 hrs (hours)
Experimental Results: Induction of apoptosis in SK-MEL-5 cells.
References
[1]. Farnaby W, et al. BAF complex vulnerabilities in cancer demonstrated via structure-based PROTAC design. Nat Chem Biol. 2019 Jul;15(7):672-680.
These protocols are for reference only. InvivoChem does not independently validate these methods.
Physicochemical Properties
Molecular Formula
C49H58FN9O7S
Molecular Weight
936.1043
CAS #
2375564-55-7
SMILES
S1C([H])=NC(C([H])([H])[H])=C1C1C([H])=C([H])C(=C(C=1[H])OC([H])([H])C([H])([H])OC1C([H])=C([H])C(=C([H])C=1[H])C([H])([H])N1C([H])([H])C([H])([H])N(C2C(N([H])[H])=NN=C(C3=C([H])C([H])=C([H])C([H])=C3O[H])C=2[H])C([H])([H])C1([H])[H])C([H])([H])N([H])C([C@]1([H])C([H])([H])[C@]([H])(C([H])([H])N1C([C@]([H])(C(C([H])([H])[H])(C([H])([H])[H])C([H])([H])[H])N([H])C(C1(C([H])([H])C1([H])[H])F)=O)=O)O[H])=O
Storage

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

Shipping Condition
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
Solubility Data
Solubility (In Vitro)
DMSO : ≥ 100 mg/mL (~106.83 mM)
Preparing Stock Solutions 1 mg 5 mg 10 mg
1 mM 1.0683 mL 5.3413 mL 10.6826 mL
5 mM 0.2137 mL 1.0683 mL 2.1365 mL
10 mM 0.1068 mL 0.5341 mL 1.0683 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.

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