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| 5mg |
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| 25mg |
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| 50mg |
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| 100mg |
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Purity: ≥98%
Adenosine Dialdehyde (also named as ADOX), an adenosine analog, is an inhibitor of the S-adenosylmethionine-dependent methyltransferase with an IC50 of 40 nM. Adenosine dialdehyde functions as a cell-incorporated indirect inhibitor. S-adenosyl-L-homo cystein (Adoicy), a product inhibitor of methyltransferases that use S-adenosyl-L-methionine (AdoMet) as the methyl group donor, can accumulate as a result of ADOX's inhibition of S-adenosyl-L-homocystein hydrolase. p53 was reactivated and p53 target genes were induced as a result of ADOX's inhibition of the Tax-activated NF-κB pathway.
| Targets |
AdoHcy hydrolase ( IC50 = 40 nM )
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| ln Vitro |
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| ln Vivo |
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| Cell Assay |
Hela cells are cultured in MEM medium with 10% fetal bovine serum supplement in an incubator with 5% CO2 at 37 °C. AdOx treatment of cells was carried out for varied lengths of time. Following cell harvesting, the cells are rinsed in phosphate-buffered saline and then resuspended in buffer A, which contains complete protease inhibitor cocktail, 5% glycerol, 1 mM sodium EGTA, 1 mM dithiothreitol, and 0.5% Triton X-100.
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| Animal Protocol |
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| References |
| Molecular Formula |
C10H11N5O4
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| Molecular Weight |
265.23
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| Exact Mass |
265.08
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| CAS # |
34240-05-6
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| Related CAS # |
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| Appearance |
Oil
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| SMILES |
C1=NC(=C2C(=N1)N(C=N2)C(C=O)OC(CO)C=O)N
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| InChi Key |
ILMNSCQOSGKTNZ-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C10H11N5O4/c11-9-8-10(13-4-12-9)15(5-14-8)7(3-18)19-6(1-16)2-17/h1,3-7,17H,2H2,(H2,11,12,13)
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| Chemical Name |
2-[1-(6-aminopurin-9-yl)-2-oxoethoxy]-3-hydroxypropanal
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| Synonyms |
Adenosine dialdehyde
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
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| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (7.84 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 2.08 mg/mL (7.84 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.7703 mL | 18.8516 mL | 37.7031 mL | |
| 5 mM | 0.7541 mL | 3.7703 mL | 7.5406 mL | |
| 10 mM | 0.3770 mL | 1.8852 mL | 3.7703 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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