Size | Price | Stock | Qty |
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50mg |
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100mg |
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500mg |
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Other Sizes |
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ln Vitro |
When treating infections brought on by germs resistant to other aminoglycosides, amikacin is unquestionably superior. Relatively few enzymes that alter aminoglycosides have an impact on amikacin. Amikacin is used to treat infections brought on by Mycobacterium avium intracellulare, Nocardia asteroides, and a few other "fast-growing" mycobacteria, such as Mycobacterium fortuitum and Mycobacterium chelonae [1]. With an LD50 value of 453 μM, amikacin (100-1500 μM) consistently causes dose-dependent loss of zebrafish lateral line hair cells [3].
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ln Vivo |
Treatment with amikacin (320 mg/kg; subcutaneous injection; daily; for 10 days; male Fischer rats) increases the chance of severe hearing loss in rats [3].
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Animal Protocol |
Animal/Disease Models: Male Fischer 344 rats (40-50 days old) [3]
Doses: 320 mg/kg Route of Administration: subcutaneous injection; daily; lasting for 10 days. Experimental Results: Caused hearing loss in rats. |
References |
[1]. Edson, R.S. and C.L. Terrell, The aminoglycosides. Mayo Clin Proc, 1999. 74(5): p. 519-28.
[2]. Ristuccia AM, et al. An overview of amikacin. Ther Drug Monit. 1985;7(1):12-25. [3]. Siân R Kitcher, et al. ORC-13661 Protects Sensory Hair Cells From Aminoglycoside and Cisplatin Ototoxicity. JCI Insight. 2019 Aug 8;4(15):e126764. |
Molecular Formula |
C22H43N5O13.H2O
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Molecular Weight |
603.618
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CAS # |
1257517-67-1
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Related CAS # |
Amikacin disulfate;39831-55-5;Amikacin sulfate;149022-22-0;Amikacin;37517-28-5
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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SMILES |
NCC[C@H](O)C(N[C@@H]1C[C@H](N)[C@@H](O[C@@H]2[C@H](O)[C@@H](O)[C@H](O)[C@H](O2)CN)[C@H](O)[C@H]1O[C@@H]3[C@H](O)[C@@H](N)[C@H](O)[C@H](O3)CO)=O.O
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
H2O : ~100 mg/mL (~244.79 mM)
DMSO :< 1 mg/mL |
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Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80: Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 :Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 1.6567 mL | 8.2834 mL | 16.5667 mL | |
5 mM | 0.3313 mL | 1.6567 mL | 3.3133 mL | |
10 mM | 0.1657 mL | 0.8283 mL | 1.6567 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.