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1g |
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5g |
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10g |
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Other Sizes |
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Purity: ≥98%
Amitriptyline (Elavil; Tryptanol; Amitriptylin; Damilen; Triptanol), a tricyclic antidepressant (TCA), is an inhibitor of serotonin receptor, norepinephrine receptor, 5-HT4, 5-HT2 and sigma 1 receptor with potential anti-depressive activity. It inhibits the sigma 1 receptor, 5-HT4, 5-HT2, norepinephrine receptor, and serotonin receptor with IC50s of 3.45 nM, 13.3 nM, 7.31 nM, 235 nM and 287 nM, respectively.
Targets |
5-HT1A Receptor ( IC50 = 450 nM ); 5-HT1B Receptor ( IC50 = 40 nM ); 5-HT2A Receptor ( IC50 = 4 nM ); 5-HT2B Receptor ( IC50 = 40 nM );
5-HT2C Receptor ( IC50 = 6 nM ); H1 Receptor ( IC50 = 1.1 nM ); H3 Receptor ( IC50 = 1 μM ); H4 Receptor ( IC50 = 33.6 nM ); SERT ( Ki = 3.45 nM ); NET ( Ki = 13.3 nM ); DAT ( Ki = 2.58 μM ); Adrenergic receptor ( IC50 = 24 nM ); muscarinic receptor ( IC50 = 7.2 nM ); TrkA; TrkB |
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ln Vitro |
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ln Vivo |
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Cell Assay |
Cell Line: hippocampal neurons
Concentration: 0.5 μM Incubation Time: 30 min Result: Induced TrkA phosphorylation. Induced Erk 1/2 and Akt signalings activation. |
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Animal Protocol |
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References |
[1]. Biol Psychiatry . 2004 Feb 1;55(3):320-2. [2]. Mol Pharmacol . 2001 Mar;59(3):427-33. [3]. J Pharmacol Exp Ther . 2001 Oct;299(1):83-9. [4]. Exp Neurol . 2007 Oct;207(2):248-57. [5]. J Pharmacol Exp Ther . 2010 Jan;332(1):255-65. [6]. Chem Biol . 2009 Jun 26;16(6):644-56. [7]. J Pharmacol Exp Ther . 2005 Nov;315(2):764-70. |
Molecular Formula |
C20H24CLN
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Molecular Weight |
313.86
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Exact Mass |
277.18
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Elemental Analysis |
C, 76.54; H, 7.71; Cl, 11.29; N, 4.46
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CAS # |
549-18-8
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Related CAS # |
Amitriptyline-d3 hydrochloride; 342611-00-1; Amitriptyline-d6 hydrochloride; 203645-63-0; Amitriptyline; 50-48-6
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Appearance |
Solid powder
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SMILES |
CN(C)CCC=C1C2=CC=CC=C2CCC3=CC=CC=C31.Cl
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InChi Key |
KFYRPLNVJVHZGT-UHFFFAOYSA-N
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InChi Code |
InChI=1S/C20H23N.ClH/c1-21(2)15-7-12-20-18-10-5-3-8-16(18)13-14-17-9-4-6-11-19(17)20;/h3-6,8-12H,7,13-15H2,1-2H3;1H
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Chemical Name |
N,N-dimethyl-3-(2-tricyclo[9.4.0.03,8]pentadeca-1(15),3,5,7,11,13-hexaenylidene)propan-1-amine;hydrochloride
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Synonyms |
trade names: Elavil; Amitriptylin; Tryptanol; Damilen; Triptanol; Amitriptyline HCl; Amitriptyline hydrochloride
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
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Solubility (In Vivo) |
Solubility in Formulation 1: 120 mg/mL (382.34 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
Solubility in Formulation 2: 5%DMSO + Corn oil: 4.0mg/ml (12.74mM)  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 3.1861 mL | 15.9307 mL | 31.8613 mL | |
5 mM | 0.6372 mL | 3.1861 mL | 6.3723 mL | |
10 mM | 0.3186 mL | 1.5931 mL | 3.1861 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
NCT Number | Recruitment | interventions | Conditions | Sponsor/Collaborators | Start Date | Phases |
NCT06158230 | Active Recruiting |
Drug: Pizotifen Drug: Amitriptyline-propranolol |
Migraine | Bangabandhu Sheikh Mujib Medical University, Dhaka, Bangladesh |
March 19, 2023 | Phase 2 |
NCT03324035 | Active Recruiting |
Drug: Amitriptyline Drug: Tramadol |
Pain, Neuropathic Leprosy |
University of Sao Paulo | March 1, 2017 | Phase 3 |
NCT04725383 | Recruiting | Drug: amitriptyline | Autism Spectrum Disorder Repetitive Compulsive Behavior |
University of Missouri, Kansas City | November 1, 2023 | Phase 3 |
NCT06162819 | Not yet recruiting | Drug: Amitriptyline Drug: Flunarizine 5mg |
Migraine Prophylaxis |
Shalamar Institute of Health Sciences | January 1, 2024 | Not Applicable |
NCT05889624 | Recruiting | Drug: Amitriptyline Behavioral: CBT |
Headache Migraine |
Children's Hospital Medical Center, Cincinnati |
August 22, 2023 | Not Applicable |
Amitriptyline activates the TrkA receptor and its downstream signaling cascades. Chem Biol . 2009 Jun 26;16(6):644-56. td> |
Amitriptyline provokes neurite outgrowth in PC12 cells. Chem Biol . 2009 Jun 26;16(6):644-56. td> |
Amitriptyline prevents neurons from apoptosis in a TrkA-dependent manner. Chem Biol . 2009 Jun 26;16(6):644-56. td> |