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1mg |
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5mg |
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10mg |
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25mg |
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Amyloid β-Peptide (1-42) human is a human form of amyloid β-peptide composed of 42-amino acids and can be found in the brains of patients with Alzheimer's disease. It plays a key role during the pathogenesis of Alzheimer disease (AD).
ln Vitro |
Recommendations for Beta-Amyloid Aggregation (This is our suggested approach; it is merely a guideline and can be adjusted to meet your unique requirements). 1. In cold hexafluoro-2-propanol (HFIP), dissolve the solid Aβ peptide. For the peptide to establish conjugation and structural randomization, let it on the shelf for a minimum of one hour. 2. Evaporate the HFIP to remove the resultant peptide, then store it in thin film at -20°C or -80°C. 3. Spin-dilute the resulting membrane to the proper concentration and buffer (serum- and phenol red-free media) after dissolving it in 5 mM anhydrous DMSO. 4. Then, for 48 hours, keep the solution between 4 and 8°C. After centrifuging the samples for 10 minutes at 4–8°C at 14,000 g, the supernatant contained soluble low loading. For the tests, the supernatant gradient 10-200 times was utilized. Different approaches are used based on the final application. Note: It is advised to use the aggregated form right away if it is unstable in solution.
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ln Vivo |
Alzheimer's disease models in animals can be created using human TFA and β-Amyloid (1-42).
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References |
[1]. Solntseva EI, et al. Impact of amyloid-β peptide (1-42) on voltage-gated ion currents in molluscan neurons. Bull Exp Biol Med. 2011 Oct;151(6):671-4.
[2]. Barucker C, et al. Nuclear translocation uncovers the amyloid peptide Aβ42 as a regulator of gene transcription. J Biol Chem. 2014 Jul 18;289(29):20182-91. [3]. Stefania Sabella, et al. Capillary electrophoresis studies on the aggregation process of beta-amyloid 1-42 and 1-40 peptides. Electrophoresis. 2004 Oct;25(18-19):3186-94. |
Molecular Formula |
C₂₀₃H₃₁₁N₅₅O₆₀S
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Molecular Weight |
4514.04
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CAS # |
107761-42-2
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Sequence |
Asp-Ala-Glu-Phe-Arg-His-Asp-Ser-Gly-Tyr-Glu-Val-His-His-Gln-Lys-Leu-Val-Phe-Phe-Ala-Glu-Asp-Val-Gly-Ser-Asn-Lys-Gly-Ala-Ile-Ile-Gly-Leu-Met-Val-Gly-Gly-Val-Val-Ile-Ala
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SequenceShortening |
DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA
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Appearance |
Typically exists as solids (or liquids in special cases) at room temperature
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SMILES |
NCCCCCC(NCC(NCC(NCC(NCC(NCC(NCC(NCC(NCC(NCC(NCC(NCC(NCC(NCC(NCC(NCC(NCC(N)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O
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Synonyms |
β-Amyloid (1-42, human
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product is not stable in solution, please use freshly prepared working solution for optimal results. (2). Please store this product in a sealed and protected environment, avoid exposure to moisture. |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : ~33.33 mg/mL (~7.20 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (0.54 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 2.5 mg/mL (0.54 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (0.54 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 0.2215 mL | 1.1077 mL | 2.2153 mL | |
5 mM | 0.0443 mL | 0.2215 mL | 0.4431 mL | |
10 mM | 0.0222 mL | 0.1108 mL | 0.2215 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.