| Size | Price | Stock | Qty |
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| 10mg |
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| 25mg |
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| 50mg |
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| 100mg |
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| Other Sizes |
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| ln Vitro |
Isopsoralen treatment stimulates the accumulation of cartilage nodules in a dose-dependent manner. Isopsoralen increases the expression of chondrogenic marker genes like collagen II, collagen X, OCN, Smad4 and Sox9 in a time-dependent manner. Additionally, isopsoralen induces the activation of p38 MAP kinase and extracellular signal-regulated kinase (ERK), but not of c-jun N-terminal kinase (JNK). Isopsoralen significantly increases BMP-2 protein expression in a time-dependent manner and, through BMP-2 or MAPK signaling pathways, mediates a chondromodulating effect.
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| ln Vivo |
In mice with sex hormone deprivation, isopsoralen has a significant osteoprotective effect on both male and female mice. Isopsoralen treatment for 8 weeks results in improved trabecular bone microstructure and increased bone strength. The elimination half-life of isopsoralen after intravenous administration to wistar rats is 5.35 hours. Kidney > lung > liver > heart > spleen > brain is the order in which the area under the tissues' curves for isopsoralen decrease. The elimination half-life of isopsoralen after oral administration to Wistar rats is 5.56 h, and its bioavailability is 70.35%.
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| Cell Assay |
ATDC5 cells are plated in 96-well plates at a density of 5×103 cells per well, incubated for an overnight period in media containing 10% FBS, and then subjected to various isopsoralen concentrations. Following a 24-hour incubation period, cells are treated with media containing 100 g/ml MTT for 2 hours at 37°C after being washed with phosphate-buffered saline (PBS). Following a PBS wash, the cells are dissolved in 200 l of DMSO. By using a spectrophotometer to measure the absorbance at a wavelength of 540 nm, the resulting solubilized purple formazan is quantified.
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| References |
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| Molecular Formula |
C11H6O3
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|---|---|
| Molecular Weight |
186.16354
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| Exact Mass |
296.09833
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| Elemental Analysis |
C, 68.89; H, 5.44; N, 9.45; O, 5.40; S, 10.82
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| CAS # |
523-50-2
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| Related CAS # |
29462-18-8; 29462-19-9 (HCl)
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| Appearance |
Solid powder
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| SMILES |
C1=CC2=C(C=CO2)C3=C1C=CC(=O)O3
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| InChi Key |
XDROKJSWHURZGO-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C11H6O3/c12-10-4-2-7-1-3-9-8(5-6-13-9)11(7)14-10/h1-6H
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| Chemical Name |
furo[2,3-h]chromen-2-one
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| Synonyms |
Knoll brand of bentazepam; QM 6008; QM-6008; Bentazepam; Thiadipone; Tiadipone; Bentazepam [USAN:INN]; CI-718; Tiadipona; Thiadipone
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 14~33.3 mg/mL (75.2~179.0 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (13.43 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (13.43 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.3717 mL | 26.8586 mL | 53.7172 mL | |
| 5 mM | 1.0743 mL | 5.3717 mL | 10.7434 mL | |
| 10 mM | 0.5372 mL | 2.6859 mL | 5.3717 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
| NCT Number | Recruitment | interventions | Conditions | Sponsor/Collaborators | Start Date | Phases |
| NCT01931436 | Completed | Drug: Qing'E pill | Menopause | Shanghai University of Traditional Chinese Medicine |
August 2013 | Phase 1 |
Products are for research use only; We do not sell to patients
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