| Size | Price | Stock | Qty |
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| 25mg |
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Purity: ≥98%
Apigetrin (Apigenin-7-O-β-D-glucopyranoside; Cosmosiin; Apigenin 7-glucoside) is a naturally occuring flavanoid derived from dandelions and exhibits significant anti-proliferative and antioxidant activity, scavengers of ROS. It may inhibit neuroinflammation in BV-2 microglia and have a neuroprotective effect in HT22 hippocampal cells. This molecule may also hinder cancer progression by inhibiting various pathways such as STAT3/JAK2.
| Targets |
ROS/reactive oxygen species
|
|---|---|
| ln Vitro |
After 24 and 48 hours of incubation, apigenin 7-glucoside demonstrated strong antiproliferative action on B16F10 melanoma cells. The number of cells in the subG0/G1, S, and G2/M phases increased as a result of apigenin-7-glucoside, whereas the fraction of cells in the G0/G1 phase significantly decreased. In B16F10 melanoma cells, apigenin-7-glucoside increases tyrosinase activity, melanogenesis, and production [1]. Specifically, apigenin 7-glucoside inhibits myeloid differentiation while inducing CD34+ cells to differentiate toward the erythroid lineage. Strong antioxidant action against reactive oxygen species (ROS) is exhibited by apigenin 7-glucoside in vitro in a concentration-dependent manner [2].
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| Cell Assay |
In this study, researchers have investigated the effects of apigenin-7-glucoside, genkwanin and naringenin, on mouse melanoma B16F10 cell proliferation. Influence of these natural products on percentage cell distribution in cycle phases and melanogenesis was also studied.
Cell viability was determined at various periods using the MTT assay, whereas effects of tested compounds on progression through the cell cycle were analyzed by flow cytometry. In addition, amounts of melanin and tyrosinase were measured spectrophotometrically at 475 nm. Besides, the mechanism involved on the death route induced by the tested molecules was evaluated using the bis-benzimide trihydrochloride coloration method (Hoechst 33258)[1]. |
| References |
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| Additional Infomation |
Apigenin 7-O-beta-D-glucoside is a glycosyloxyflavone that is apigenin substituted by a beta-D-glucopyranosyl moiety at position 7 via a glycosidic linkage. It has a role as a non-steroidal anti-inflammatory drug, a metabolite and an antibacterial agent. It is a beta-D-glucoside, a dihydroxyflavone, a glycosyloxyflavone and a monosaccharide derivative. It is functionally related to an apigenin. It is a conjugate acid of an apigenin 7-O-beta-D-glucoside(1-). It is an enantiomer of an apigenin 7-O-beta-L-glucoside.
Cosmosiin has been reported in Acanthus ebracteatus, Coreopsis lanceolata, and other organisms with data available. See also: Chamomile (part of). |
| Molecular Formula |
C21H20O10
|
|---|---|
| Molecular Weight |
432.3775
|
| Exact Mass |
432.105
|
| Elemental Analysis |
C, 58.34; H, 4.66; O, 37.00
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| CAS # |
578-74-5
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| Related CAS # |
Apigenin;520-36-5
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| PubChem CID |
5280704
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| Appearance |
Light yellow to yellow solid powder
|
| Density |
1.6±0.1 g/cm3
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| Boiling Point |
788.9±60.0 °C at 760 mmHg
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| Melting Point |
230-237ºC
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| Flash Point |
280.7±26.4 °C
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| Vapour Pressure |
0.0±2.9 mmHg at 25°C
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| Index of Refraction |
1.717
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| LogP |
-0.39
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| Hydrogen Bond Donor Count |
6
|
| Hydrogen Bond Acceptor Count |
10
|
| Rotatable Bond Count |
4
|
| Heavy Atom Count |
31
|
| Complexity |
675
|
| Defined Atom Stereocenter Count |
5
|
| SMILES |
C1=CC(=CC=C1C2=CC(=O)C3=C(C=C(C=C3O2)O[C@H]4[C@@H]([C@H]([C@@H]([C@H](O4)CO)O)O)O)O)O
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| InChi Key |
KMOUJOKENFFTPU-OBJCFNGXSA-N
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| InChi Code |
1S/C21H20O10/c22-8-16-18(26)19(27)20(28)21(31-16)29-11-5-12(24)17-13(25)7-14(30-15(17)6-11)9-1-3-10(23)4-2-9/h1-7,16,18-24,26-28H,8H2/t16-,18+,19+,20-,21-/m1/s1
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| Chemical Name |
7-(beta-D-Glucopyranosyloxy)-5-hydroxy-2-(4-hydroxyphenyl)-4H-1-benzopyran-4-one
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| Synonyms |
Apigenin-7-O-β-D-glucopyranoside; Cosmosiin; Apigetrin; COSMOSIIN; Apigenin 7-glucoside; Cosmosiine; Cosmosioside; APIGENIN-7-GLUCOSIDE; Apigenin 7-O-beta-D-glucoside; Apigenin 7-glucoside
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ≥ 100 mg/mL (~231.28 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (5.78 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (5.78 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.3128 mL | 11.5639 mL | 23.1278 mL | |
| 5 mM | 0.4626 mL | 2.3128 mL | 4.6256 mL | |
| 10 mM | 0.2313 mL | 1.1564 mL | 2.3128 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
| NCT Number | Recruitment | interventions | Conditions | Sponsor/Collaborators | Start Date | Phases |
| NCT04873349 | Completed | Dietary Supplement: Nusapure standardized olive leaves capsule, 750 mg (50% oleuropein) |
COVID-19 Acute Bronchitis COVID-19 Respiratory Infection Covid19 Disseminated Intravascular Coagulation Symptoms and Signs |
Shimaa M. Abdelgawad | 2021-05-10 | Not Applicable |
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